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Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry
Comparison of the HMS-PCI data set with interactions reported in the literature revealed an average threefold higher success rate in detection of known complexes compared with large-scale two-hybrid studies. Expand
Early lineage segregation between epiblast and primitive endoderm in mouse blastocysts through the Grb2-MAPK pathway.
It is shown that early inner cell mass (ICM) at E3.5 is already heterogeneous, and a model is proposed in which the ICM develops as a mosaic of EPI and PE progenitors at E 3.5, dependent on Grb2-Ras-MAP kinase signaling, followed by later segregation of the progenitor into the appropriate cell layers. Expand
Dissecting virulence: systematic and functional analyses of a pathogenicity island.
- W. Deng, J. Puente, +12 authors B. Finlay
- Biology, Medicine
- Proceedings of the National Academy of Sciences…
- 9 March 2004
This work systematically mutagenized all 41 CR LEE genes and functionally characterized these mutants in vitro and in a murine infection model, identifying 33 virulence factors, including two virulence regulators and a hierarchical switch for type III secretion. Expand
A mammalian PAR-3–PAR-6 complex implicated in Cdc42/Rac1 and aPKC signalling and cell polarity
- D. Lin, A. S. Edwards, J. P. Fawcett, G. Mbamalu, John D. Scott, T. Pawson
- Biology, Medicine
- Nature Cell Biology
- 1 August 2000
It is shown that homologues of proteins necessary for asymmetric cell division in Caenorhabditis elegans associate with each other in mammalian cells and tissues and have a scaffolding function, coordinating the activities of several signalling proteins that are implicated in mammalian cell polarity. Expand
Phase Transition of a Disordered Nuage Protein Generates Environmentally Responsive Membraneless Organelles
It is demonstrated that the disordered tails of Ddx4, a primary constituent of nuage or germ granules, form phase-separated organelles both in live cells and in vitro, and proposed that phase separation of disordered proteins containing weakly interacting blocks is a general mechanism for forming regulated, membraneless organlles. Expand
Multisite phosphorylation of a CDK inhibitor sets a threshold for the onset of DNA replication
The weak CPD sites in Sic1 establish a phosphorylation threshold that delays degradation in vivo, and thereby establishes a minimal G1 phase period needed to ensure proper DNA replication. Expand
BIND: the Biomolecular Interaction Network Database
- Gary D Bader, I. Donaldson, C. Wolting, B. Ouellette, T. Pawson, C. Hogue
- Computer Science, Medicine
- Nucleic Acids Res.
A new graphical analysis tool is developed that provides users with a view of the domain composition of proteins in interaction and complex records to help relate functional domains to protein interactions in BIND. Expand
Backbone dynamics of a free and phosphopeptide-complexed Src homology 2 domain studied by 15N NMR relaxation.
Overall, higher order parameters were not found in the peptide-bound form, indicating that on average, picosecond-time-scale disorder is not reduced upon binding peptide, and the relaxation data of the SH2-phosphopeptide complex were fit with fewer exchange terms than the uncomplexed form. Expand
Histone Recognition and Large-Scale Structural Analysis of the Human Bromodomain Family
Bromodomains are protein interaction modules that specifically recognize ε-N-lysine acetylation motifs, a key event in the reading process of epigenetic marks, and a structural mechanism for the simultaneous binding and recognition of diverse diacetyl-containing peptides by BRD4 is uncovered. Expand
Repulsive Axon Guidance Abelson and Enabled Play Opposing Roles Downstream of the Roundabout Receptor
Genetic and biochemical evidence is presented that the Abelson (Abl) tyrosine kinase and its substrate Enabled (Ena) play direct and opposing roles in Robo signal transduction. Expand