Share This Author
Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry
Comparison of the HMS-PCI data set with interactions reported in the literature revealed an average threefold higher success rate in detection of known complexes compared with large-scale two-hybrid studies.
Early lineage segregation between epiblast and primitive endoderm in mouse blastocysts through the Grb2-MAPK pathway.
BIND: the Biomolecular Interaction Network Database
- Gary D Bader, I. Donaldson, Cheryl Wolting, B. F. Ouellette, T. Pawson, C. Hogue
- Biology, Computer ScienceNucleic Acids Res.
- 1 January 2001
A new graphical analysis tool is developed that provides users with a view of the domain composition of proteins in interaction and complex records to help relate functional domains to protein interactions in BIND.
Backbone dynamics of a free and phosphopeptide-complexed Src homology 2 domain studied by 15N NMR relaxation.
Overall, higher order parameters were not found in the peptide-bound form, indicating that on average, picosecond-time-scale disorder is not reduced upon binding peptide, and the relaxation data of the SH2-phosphopeptide complex were fit with fewer exchange terms than the uncomplexed form.
Histone Recognition and Large-Scale Structural Analysis of the Human Bromodomain Family
A mammalian PAR-3–PAR-6 complex implicated in Cdc42/Rac1 and aPKC signalling and cell polarity
- D. Lin, A. S. Edwards, J. P. Fawcett, G. Mbamalu, John D. Scott, T. Pawson
- BiologyNature Cell Biology
- 1 August 2000
It is shown that homologues of proteins necessary for asymmetric cell division in Caenorhabditis elegans associate with each other in mammalian cells and tissues and have a scaffolding function, coordinating the activities of several signalling proteins that are implicated in mammalian cell polarity.
Phase Transition of a Disordered Nuage Protein Generates Environmentally Responsive Membraneless Organelles
Dissecting virulence: systematic and functional analyses of a pathogenicity island.
- W. Deng, J. Puente, B. Finlay
- Biology, MedicineProceedings of the National Academy of Sciences…
- 9 March 2004
This work systematically mutagenized all 41 CR LEE genes and functionally characterized these mutants in vitro and in a murine infection model, identifying 33 virulence factors, including two virulence regulators and a hierarchical switch for type III secretion.
Multisite phosphorylation of a CDK inhibitor sets a threshold for the onset of DNA replication
The weak CPD sites in Sic1 establish a phosphorylation threshold that delays degradation in vivo, and thereby establishes a minimal G1 phase period needed to ensure proper DNA replication.