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The Conserved Noncatalytic 40-Residue Sequence in Cellulases and Hemicellulases from Anaerobic Fungi Functions as a Protein Docking Domain (*)
Two cDNAs, designated xynA and manA, encoding xylanase A (XYLA) and mannanase A (MANA), respectively, were isolated from a cDNA library derived from mRNA extracted from the anaerobic fungus,Expand
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Trp22, Trp24, and Tyr8 play a pivotal role in the binding of the family 10 cellulose-binding module from Pseudomonas xylanase A to insoluble ligands.
Aromatic amino acids are believed to play a pivotal role in carbohydrate-binding proteins, by forming hydrophobic stacking interactions with the sugar rings of their target ligands. Family 10Expand
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An effective and reproducible transformation protocol for the model resurrection plant Craterostigma plantagineum Hochst.
Abstract. Procedures previously established for plant regeneration and Agrobacterium tumefaciens-mediated genetic transformation of the desiccation-tolerant plant, Craterostigma plantagineum, haveExpand
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Interaction of Azospirillum lipoferum with wheat germ agglutinin stimulates nitrogen fixation.
In vitro, the nitrogen fixation capability of A. lipoferum is efficiently increased in the presence of wheat germ agglutinin (WGA). A putative WGA-binding receptor, a 32-kDa protein, was detected inExpand
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Identification of cohesive ends and genes encoding the terminase of phage 16-3.
Cohesive ends of 16-3, a temperate phage of Rhizobium meliloti 41, have been identified as 10-base-long, 3'-protruding complementary G/C-rich sequences. terS and terL encode the two subunits of 16-3Expand
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Rapid screening for endo-β-1,4-glucanase and endo-β-1,4-mannanase activities and specific measurement using soluble dye-labelled substrates
Soluble dye-labelled substrates were prepared for the specific and rapid screening and the measurement of endo-β-1,4-glucanase and endo-β-1,4-mannanase activities. Soluble carboxy-methylcellulose andExpand
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immX immunity region of rhizobium phage 16-3: two overlapping cistrons of repressor function.
16-3 is a temperate phage of the symbiotic nitrogen-fixing bacterium Rhizobium meliloti 41. Its prophage state and immunity against superinfection by homoimmune phages are governed by a complex setExpand
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Artificial tripartite symbiosis involving a green alga (Chlamydomonas), a bacterium (Azotobacter) and a fungus (Alternaria): Morphological and physiological characterization
A long-living artificial tripartite symbiosis involving a green alga (Chlamydomonas), a bacterium (Azotobacter) and a fungus (Alternaria) was established on carbon- and nitrogen-free medium. TheExpand
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A new approach for the biolistic method: Bombardment of living nitrogen-fixing bacteria into plant tissues
SummaryA new utilization of the biolistic gun was developed for the direct introduction of nitrogen-fixing bacteria (Azotobacter vinelandii) into strawberry (Fragaria x ananassa) tissues. This wasExpand
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Cloning and expression of a β-1,4-endoglucanase gene from Cellulomonas sp. CelB7 in Escherichia coli; purification and characterization of the recombinant enzyme
A gene library of a newly isolated Cellulomonas sp. strain was constructed in Escherichia coli and clones were screened for endoglucanase activity using dye-labelled carboxymethylcellulose. SeventeenExpand
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