T. Ojima

Publications158
h-index25
Citations2,260
Highly Influential Citations79
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Purification and cDNA cloning of a cellulase from abalone Haliotis discus hannai.
A cellulase [endo-beta-1,4-D-glucanase (EC 3.2.1.4)] was isolated from the hepatopancreas of abalone Haliotis discus hannai by successive chromatographies on TOYOPEARL CM-650M, hydroxyapatite and… Expand
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Characterization of an Alginate Lyase, FlAlyA, from Flavobacterium sp. Strain UMI-01 and Its Expression in Escherichia coli
A major alginate lyase, FlAlyA, was purified from the periplasmic fraction of an alginate-assimilating bacterium, Flavobacterium sp. strain UMI-01. FlAlyA showed a single band of ~30 kDa on SDS-PAGE… Expand
  • 52
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Comparative studies on the functional roles of N‐ and C‐terminal regions of molluskan and vertebrate troponin‐I
Vertebrate troponin regulates muscle contraction through alternative binding of the C‐terminal region of the inhibitory subunit, troponin‐I (TnI), to actin or troponin‐C (TnC) in a Ca2+‐dependent… Expand
  • 23
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Enzymatic properties and primary structures of two α-amylase isozymes from the Pacific abalone Haliotis discus hannai.
Two α-amylase (EC 3.2.1.1) isozymes, HdAmy58 and HdAmy82, with approximate molecular masses of 58 kDa and 82 kDa, respectively, were isolated from the digestive fluid of the Pacific abalone Haliotis… Expand
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Amino acid sequence of troponin C from scallop striated adductor muscle.
Troponin and its Ca(2+)-binding subunit troponin C (TnC) of the Ezo-giant scallop, Patinopecten yessoensis, have been revealed to bind only 1 mol of Ca2+/mol irrespective of the presence and absence… Expand
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Isolation and primary structure of a cellulase from the Japanese sea urchin Strongylocentrotus nudus.
Glycoside-hydrolase-family 9 (GHF9) cellulases are known to be widely distributed in metazoa. These enzymes have been appreciably well investigated in protostome invertebrates such as arthropods,… Expand
  • 88
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Isolation and characterization of two types of beta-1,3-glucanases from the common sea hare Aplysia kurodai.
  • Yuya Kumagai, T. Ojima
  • Biology, Medicine
  • Comparative biochemistry and physiology. Part B…
  • 1 February 2010
Two types of beta-1,3-glucanases, AkLam36 and AkLam33 with the molecular masses of 36kDa and 33kDa, respectively, were isolated from the digestive fluid of the common sea hare Aplysia kurodai.… Expand
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Structural basis for controlling the enzymatic properties of polymannuronate preferred alginate lyase FlAlyA from the PL-7 family.
FlAlyA is an endolytic enzyme with a preference for polymannuronate. The crystal structure and mutagenesis studies elucidated that the structural variations at outer uronate-binding subsites +2, +3… Expand
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An endo-beta-1,4-mannanase, AkMan, from the common sea hare Aplysia kurodai.
A mannan-degrading enzyme was isolated from the digestive fluid of the common sea hare Aplysia kurodai by ammonium sulfate fractionation followed by conventional column chromatography. The purified… Expand
  • 26
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A Novel Aldo-Keto Reductase, HdRed, from the Pacific Abalone Haliotis discus hannai, Which Reduces Alginate-derived 4-Deoxy-l-erythro-5-hexoseulose Uronic Acid to 2-Keto-3-deoxy-d-gluconate*
Abalone feeds on brown seaweeds and digests seaweeds' alginate with alginate lyases (EC 4.2.2.3). However, it has been unclear whether the end product of alginate lyases (i.e. unsaturated… Expand
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