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The RGD motif in fibronectin is essential for development but dispensable for fibril assembly
Fibronectin (FN) is secreted as a disulfide-bonded FN dimer. Each subunit contains three types of repeating modules: FN-I, FN-II, and FN-III. The interactions of α5β1 or αv integrins with the RGDExpand
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An experimental study of GFP‐based FRET, with application to intrinsically unstructured proteins
We have experimentally studied the fluorescence resonance energy transfer (FRET) between green fluorescent protein (GFP) molecules by inserting folded or intrinsically unstructured proteins betweenExpand
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Irisin – a myth rather than an exercise-inducible myokine
The myokine irisin is supposed to be cleaved from a transmembrane precursor, FNDC5 (fibronectin type III domain containing 5), and to mediate beneficial effects of exercise on human metabolism.Expand
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Dynamics and elasticity of the fibronectin matrix in living cell culture visualized by fibronectin-green fluorescent protein.
Fibronectin (FN) forms the primitive fibrillar matrix in both embryos and healing wounds. To study the matrix in living cell cultures, we have constructed a cell line that secretes FN moleculesExpand
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The Structure of Irisin Reveals a Novel Intersubunit β-Sheet Fibronectin Type III (FNIII) Dimer
Background: Irisin, which corresponds to the FNDC5 receptor ectodomain, is a purported exercise-induced myokine produced by cleavage. Results: Irisin forms an extensive intersubunit β-sheet dimerExpand
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Dual labeling of the fibronectin matrix and actin cytoskeleton with green fluorescent protein variants.
We have prepared 3T3 cells doubly labeled to visualize simultaneously the extracellular fibronectin (FN) matrix and intracellular actin cytoskeleton in living cell cultures. We used FN-yellowExpand
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Structural evidence that the P/Q domain of ZipA is an unstructured, flexible tether between the membrane and the C-terminal FtsZ-binding domain.
The cell division protein ZipA has an N-terminal transmembrane domain and a C-terminal globular domain that binds FtsZ. Between them are a charged domain and a P/Q domain rich in proline andExpand
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How the kinetochore couples microtubule force and centromere stretch to move chromosomes
The Ndc80 complex (Ndc80, Nuf2, Spc24 and Spc25) is a highly conserved kinetochore protein essential for end-on anchorage to spindle microtubule plus ends and for force generation coupled to plus-endExpand
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Oligomeric structure and tissue distribution of ficolins from mouse, pig and human.
Mouse plasma ficolin was purified by GlcNAc affinity and anion-exchange chromatography. Gel-filtration chromatography and gradient sedimentation indicated that mouse plasma ficolin is a 12-mer ofExpand
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Two Oligomeric Forms of Plasma Ficolin Have Differential Lectin Activity*
Ficolins are plasma proteins with binding activity for carbohydrates, elastin, and corticosteroids. The ficolin polypeptide has a collagen-like domain that presumably brings three subunits togetherExpand
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