Whole-Genome Sequencing of Sake Yeast Saccharomyces cerevisiae Kyokai no. 7
- T. Akao, Isao Yashiro, H. Shimoi
- BiologyDNA Research
- 6 September 2011
The draft whole-genome shotgun sequence of a commonly used diploid sake yeast strain, Kyokai no. 7 (K7), is reported, finding that two regions in chromosomes I and VII of S288C have apparently been replaced by Ty elements in K7.
Chromosomal location of Lg‐FLO1 in bottom‐fermenting yeast and the FLO5 locus of industrial yeast
- T. Ogata, M. Izumikawa, K. Kohno, K. Shibata
- Biology, ChemistryJournal of Applied Microbiology
- 1 October 2008
To determine the chromosomal location and entire sequence of Lg‐FLO1, the expression of which causes the flocculation of bottom‐fermenting yeast, and to establish the carrier and removal status of canine coronavirus.
Detection of Pectinatus spp. by PCR Using 16S-23S rDNA Spacer Regions
- Y. Motoyama, T. Ogata
- 1 January 2000
The method developed in this study was rapid and sensitive, and allowed identification at the species level, even between very closely related species, such as P. cerevisiiphilus and P. frisingensis.
Modified Multiplex PCR Methods for Comprehensive Detection of Pectinatus and Beer-Spoilage Cocci
- K. Iijima, S. Asano, Koji Suzuki, T. Ogata, Y. Kitagawa
- Medicine, BiologyBioscience, biotechnology and biochemistry
- 23 October 2008
Each modified multiplex PCR method was found specifically to detect beer-spoilage species of Pectinatus and cocci, including new species.
Construction and evaluation of self‐cloning bottom‐fermenting yeast with high SSU1 expression
Aim: To construct a self‐cloning brewer’s yeast that can minimize the unfavourable flavours caused by oxidation and certain kinds of sulfur compounds.
Chromosomal structures of bottom fermenting yeasts.
- H. Yamagishi, T. Ogata
- Biology, MedicineSystematic and Applied Microbiology
- 1 September 1999
Enzymatic 2'-N-acetylation of arbekacin and antibiotic activity of its product.
It is believed 2'-N-acetyl ABK has the highest and broadest antibacterial activity, compared with known N- acetylated AGs.
Expression of the glutathione synthetase gene of Escherichia coli B in Saccharomyces cerevisiae
- Y. Ohtake, Kunihiko Watanabe, A. Kimura
- Biology, Engineering
Differentiation between brewing and non‐brewing yeasts using a combination of PCR and RFLP
- H. Yamagishi, Y. Otsuta, W. Funahashi, T. Ogata, K. Sakai
- Biology, MedicineJournal of Applied Microbiology
- 1 March 1999
It is suggested that it is possible to identify brewing from non‐brewing yeasts using specific PCR of FLO1 and rDNA, and detection of restriction fragment polymorphism of rDNA.
Construction of self‐cloning bottom‐fermenting yeast with low vicinal diketone production by the homo‐integration of ILV5
- K. Kusunoki, T. Ogata
- 1 October 2012
To shorten beer maturation time, a self‐cloning, bottom‐fermenting yeast with low VDK production is constructed by integrating ILV5, a gene encoding a protein that metabolizes α‐acetolactate andα‐aceto‐α‐hydroxybutyrate (precursors of VDK).