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Three genomes differentially contribute to the biosynthesis of benzoxazinones in hexaploid wheat.
The genomic bias in the transcription of the TaBx genes in hexaploid wheat originated in the diploid progenitors and has been retained through the polyploidization, indicating the homoeologs on the B genome generally contributed the most to the Bx biosynthesis inhexaploids wheat.
Dispersed Benzoxazinone Gene Cluster: Molecular Characterization and Chromosomal Localization of Glucosyltransferase and Glucosidase Genes in Wheat and Rye1[W]
Full sets of homeologous and paralogous genes encoding Bx glucosyltransferase (GT) and Bx-glucoside glucosidase (Glu) in hexaploid wheat and rye are identified and contribute to the understanding of the evolutionary processes that the BX-pathway loci have undergone in grasses.
Rearrangement of the genes for the biosynthesis of benzoxazinones in the evolution of Triticeae species
A cDNA of TaBx1 is isolated, which encodes the first enzyme of the Bx pathway of wheat, suggesting that the non-Bx phenotype of barley is attributable to the loss of Bx biosynthetic genes in Triticeae species.
Molecular characterization and chromosomal localization of cytochrome P450 genes involved in the biosynthesis of cyclic hydroxamic acids in hexaploid wheat
The cloned P450 species showed 76–79% identity at the amino acid level to the corresponding maize P 450 species CYP71C1–C4, which are also required for DIBOA biosynthesis, and the wheat P450 cDNAs were heterologously expressed in the yeast (Saccharomyces cerevisiae) strain AH22.
The New β-D-Glucosidase in Terpenoid-Isoquinoline Alkaloid Biosynthesis in Psychotria ipecacuanha*
Ipecac alkaloids produced in the medicinal plant Psychotria ipecacuanha such as emetine and cephaeline possess a monoterpenoid-tetrahydroisoquinoline skeleton, which is formed by condensation of
Structures of the three homoeologous loci of wheat benzoxazinone biosynthetic genes TaBx3 and TaBx4 and characterization of their promoter sequences
The results imply that stage-specific transcription ofTaBx3 and TaBx4 is not controlled by global sequence similarity of their promoters but by some essential cis-elements.
Molecular and Structural Characterization of Hexameric β-d-Glucosidases in Wheat and Rye[W]
The wheat (Triticum aestivum) and rye (Secale cereale) β-d-glucosidases hydrolyze hydroxamic acid-glucose conjugates, exist as different types of isozyme, and function as oligomers. In this study,
Molecular characterization of benzoxazinone-deficient mutation in diploid wheat.
Findings suggest that the Bx deficiency in T. boeoticum first resulted from disintegration of the TbBx1 coding sequence, followed by transcription failure, disintegrated of the coding sequences and elimination of theTbBX1-Tbx5 genes.
Three New O-Methyltransferases Are Sufficient for All O-Methylation Reactions of Ipecac Alkaloid Biosynthesis in Root Culture of Psychotria ipecacuanha
Three cDNAs, IpeOMT1–IpeT3, encoding ipecac alkaloid O-methyltransferases (OMTs) from P. ipecACuanha are identified, indicating that they are sufficient for all O- methylation reactions of ipecacs alkaloids biosynthesis.
A novel xylogenic suspension culture model for exploring lignification in Phyllostachys bamboo
The authors' xylogenic suspension culture model could be used for detailed characterization of physiological and molecular biological events in living bamboo cells.