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Engineered CRISPR-Cas9 nuclease with expanded targeting space
Expanding the targeting space of Cas9 CRISPR-Cas9 associates with a guide RNA to target and cleave a specific DNA site next to a protospacer adjacent motif (PAM). Streptococcus pyogenes Cas9Expand
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CRISPR/Cas9 mediated genome editing in ES cells and its application for chimeric analysis in mice
Targeted gene disrupted mice can be efficiently generated by expressing a single guide RNA (sgRNA)/CAS9 complex in the zygote. However, the limited success of complicated genome editing, such asExpand
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FANCD1/BRCA2 Plays Predominant Role in the Repair of DNA Damage Induced by ACNU or TMZ
Nimustine (ACNU) and temozolomide (TMZ) are DNA alkylating agents which are commonly used in chemotherapy for glioblastomas. ACNU is a DNA cross-linking agent and TMZ is a methylating agent. TheExpand
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DNA ligase IV is a potential molecular target in ACNU sensitivity
Nimustine (ACNU) is a chloroethylating agent which was the most active chemotherapy agent used for patients with high‐grade gliomas until the introduction of temozolomide, which became the standardExpand
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Repair pathways independent of the Fanconi anemia nuclear core complex play a predominant role in mitigating formaldehyde-induced DNA damage.
The role of the Fanconi anemia (FA) repair pathway for DNA damage induced by formaldehyde was examined in the work described here. The following cell types were used: mouse embryonic fibroblast cellExpand
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ATM is the predominant kinase involved in the phosphorylation of histone H2AX after heating.
Heating induces histone H2AX phosphorylation at serine 139 (gammaH2AX). Phosphorylated H2AX subsequently forms foci in numerous mammalian cell lines. The aim of this study was to clarify details inExpand
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Bi-allelic DNAH8 Variants Lead to Multiple Morphological Abnormalities of the Sperm Flagella and Primary Male Infertility.
Sperm malformation is a direct factor for male infertility. Multiple morphological abnormalities of the flagella (MMAF), a severe form of asthenoteratozoospermia, are characterized by immotileExpand
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Relationship between cyclic AMP‐dependent protein tyrosine phosphorylation and extracellular calcium during hyperactivation of boar spermatozoa
In mammalian spermatozoa, the state of protein tyrosine phosphorylation is modulated by protein tyrosine kinases and protein tyrosine phosphatases that are controlled via cyclic AMP (cAMP)‐proteinExpand
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Distinct segment‐specific functions of calyculin A‐sensitive protein phosphatases in the regulation of cAMP‐triggered events in ejaculated bull spermatozoa
Livestock spermatozoa possess more tenacious suppressors of cAMP‐triggered events—including capacitation‐associated changes—than laboratory animal spermatozoa, leading to flagellar hyperactivation.Expand
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Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing SgRNA/Cas9 Expressing Plasmids.
In mammalian cells, genome editing with the single guide RNA (sgRNA)/Cas9 complex allows for high targeting efficiency within a relatively short time frame with the added benefits of being low costExpand
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