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Polyketide synthase gene coupled to the peptide synthetase module involved in the biosynthesis of the cyclic heptapeptide microcystin.
The mcyDEFG operon is presumed to be responsible for 3-amino-9-methoxy-2,6, 8-trimethyl-10-phenyldeca-4,6-dienoic acid (Adda) biosynthesis, and the incorporation of Adda and glutamic acid into the microcystin molecule.
Genetic analysis of the peptide synthetase genes for a cyclic heptapeptide microcystin in Microcystis spp.
- T. Nishizawa, M. Asayama, K. Fujii, K. Harada, M. Shirai
- Biology, ChemistryJournal of biochemistry
- 1 September 1999
Southern hybridization and partial DNA sequencing analyses of six microcystin-producing and two non-producing Microcystis strains suggested that the microcysteine-producing strains contain the mcy gene and the non- producing strains can be divided into two groups, those possessing no mcy genes and those with m Cy genes.
Higher diversity and abundance of denitrifying microorganisms in environments than considered previously
New PCR primers covering the diverse nirK and nirS are developed and shown to be more phylogenetically diverse and 2–6 times more abundant than those revealed with the conventional primers.
Detection of betaproteobacteria inside the mycelium of the fungus Mortierella elongata.
The 16S rRNA gene was successfully amplified with DNA extracted directly from the endobacterium-containing fungal strains and all sequences were related to that of Candidatus Glomeribacter gigasporarum in the family Burkholderiaceae.
Development of PCR primers targeting fungal nirK to study fungal denitrification in the environment
Mycoavidus cysteinexigens gen. nov., sp. nov., an endohyphal bacterium isolated from a soil isolate of the fungus Mortierella elongata.
A comparative analysis of 16S rRNA gene sequences revealed that strain B1-EBT forms a distinct clade in the family Burkholderiaceae, encompassing a group of endosymbionts associated with several soil isolates of M. elongata.
Physiological Analysis of the Stringent Response Elicited in an Extreme Thermophilic Bacterium, Thermus thermophilus
- K. Kasai, T. Nishizawa, Kosaku Takahashi, T. Hosaka, H. Aoki, K. Ochi
- BiologyJournal of bacteriology
- 1 October 2006
ABSTRACT Guanosine tetraphosphate (ppGpp) is a key mediator of stringent control, an adaptive response of bacteria to amino acid starvation, and has thus been termed a bacterial alarmone. Previous…
Detection of Anammox Activity and 16S rRNA Genes in Ravine Paddy Field Soil
Results suggest that a specific population of anammox bacteria was present in paddy soils, although a small part of dinitrogen gas was emitted from the soil via anamm ox.
Molecular Analysis of the Rebeccamycin l-Amino Acid Oxidase from Lechevalieria aerocolonigenes ATCC 39243
This work provides the first analysis of a structural enzyme involved in construction of this important class of indolocarbazole natural products, and shows significant preference for 7-chloro-L-tryptophan, suggesting its likely role as the natural early pathway substrate.