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Aureusidin synthase: a polyphenol oxidase homolog responsible for flower coloration.
DNA sequence analysis revealed that aureusidin synthase belongs to the plant polyphenol oxidase family, providing an unequivocal example of the function of the polyphenl oxidase homolog in plants, i.e., flower coloration. Expand
Cold-active esterase from Psychrobacter sp. Ant300: gene cloning, characterization, and the effects of Gly-->Pro substitution near the active site on its catalytic activity and stability.
Observations could be explained in terms of a decrease in active-site flexibility brought about by the mutation and were consistent with the hypothesis that cold activity and thermolability arise from local flexibility around the active site of the enzyme. Expand
Functional Differentiation of the Glycosyltransferases That Contribute to the Chemical Diversity of Bioactive Flavonol Glycosides in Grapevines (Vitis vinifera)[W][OA]
The results presented here suggest that the sugar donor specificities of these Vv GTs could be determined by a limited number of amino acid substitutions in the primary structures of protein duplicates, illustrating the plasticity of plant glycosyltransferases in acquiring new sugar donor Specificities. Expand
A UDP-Glucose:Isoflavone 7-O-Glucosyltransferase from the Roots of Soybean (Glycine max) Seedlings
The results led to the proposal that GmIF7GT utilizes a strategy of catalysis that is distinct from those proposed for UGT71G1 and VvGT1, and suggest that an acidic residue at position 392 is very important for primary catalysis of Gmif7GT. Expand
Cloning, heterologous expression, renaturation, and characterization of a cold-adapted esterase with unique primary structure from a psychrotroph Pseudomonas sp. strain B11-1.
A gene coding for an esterase of the psychrotrophic bacterium Pseudomonas sp. Expand
Localization of a flavonoid biosynthetic polyphenol oxidase in vacuoles.
The biosynthesis of a flavonoid skeleton in vacuoles is demonstrated and may provide a strategy for overcoming the biochemical instability of the precursor chalcones in the cytoplasm, thus leading to the efficient accumulation of aurones in the flower. Expand
Identification and reconstitution of the rubber biosynthetic machinery on rubber particles from Hevea brasiliensis
It is shown that HRT1, a cPT from Heveabrasiliensis, exhibits distinct RTase activity in vitro only when it is introduced on detergent-washed HeveaRPs (WRPs) by a cell-free translation-coupled system, indicating proper introduction of cPT on RP is the key to reconstitute active RTase. Expand
Anthocyanin acyltransferases: specificities, mechanism, phylogenetics, and applications
Abstract Anthocyanins are responsible for the orange to blue coloration of flowers, fruits, and leaves. They are beneficial to human health and widely used as food colorants. AnthocyaninExpand
An isoflavone conjugate-hydrolyzing beta-glucosidase from the roots of soybean (Glycine max) seedlings: purification, gene cloning, phylogenetics, and cellular localization.
The purification and cDNA cloning of this important beta-glucosidase from the roots of G. max seedlings as well as related phylogenetic and cellular localization studies revealed that the enzyme is exclusively localized in the cell wall and intercellular space of seedling roots. Expand
Alicyclobacillus sendaiensis sp. nov., a novel acidophilic, slightly thermophilic species isolated from soil in Sendai, Japan.
An acidophilic, slightly thermophilic bacterium that produces a thermostable extracellular acid collagenase activity with potential industrial applications was isolated from soil of Aoba-yama Park, Sendai, Japan and a novel species is proposed, Alicyclobacillus sendaiensis sp. Expand