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Evaluation of a microplate DNA : DNA hybridization method compared with the initial renaturation method
It is concluded that the microplate method can be used as a reliable taxonomic tool and in very good correlation with the initial renaturation method.
Natrinema versiforme sp. nov., an extremely halophilic archaeon from Aibi salt lake, Xinjiang, China.
Comparative analysis of phenotypic properties and DNA-DNA hybridization between strain XF10T and the Natrinema species supported the conclusion that strain Xf10T is a novel species within the genus NatRinema.
Natronobacterium nitratireducens sp. nov., a aloalkaliphilic archaeon isolated from a soda lake in China.
Detailed phenotypic characterization and DNA-DNA hybridization studies revealed that the two strains belong to a new species in the genus Natronobacterium, for which the name NatronOBacterium nitratireducens sp.
Caldisphaera lagunensis gen. nov., sp. nov., a novel thermoacidophilic crenarchaeote isolated from a hot spring at Mt Maquiling, Philippines.
Four novel, thermoacidophilic, crenarchaeotic cocci that grew anaerobically and heterotrophically were isolated from an acidic hot spring in the Philippines; two representative strains were characterized in detail and phylogenetic analysis based on 16S rDNA sequences showed that the novel cocci represent an independent lineage.
Characterization and Identification ofPediococcus Species Isolated from Forage Crops and Their Application for Silage Preparation
Pediococcus species isolated from forage crops were characterized, and their application to silage preparation was studied, finding P. acidilactici LA 3 and LA 35 are considered suitable as potential silage inoculants.
Non-universal usage of the leucine CUG codon and the molecular phylogeny of the genus Candida.
This work used an in vitro cell-free translation system to identify the amino acid assignment of codon CUG in 78 species and 7 varieties of galactose-lacking Candida species equipped with Q9 as the major ubiquinone to suggest non-universal decoding is not a rare event, and that it is widely distributed in the genus Candida.
Vulcanisaeta distributa gen. nov., sp. nov., and Vulcanisaeta souniana sp. nov., novel hyperthermophilic, rod-shaped crenarchaeotes isolated from hot springs in Japan.
Seventeen strains of rod-shaped, heterotrophic, anaerobic, hyperthermophilic crenarchaeotes were isolated from several hot spring areas in eastern Japan, and eight representative strains were characterized further, revealing that they represented an independent lineage in the family Thermoproteaceae.
Distribution and identification of red yeasts in deep-sea environments around the northwest Pacific Ocean
We isolated 99 yeast strains, including 40 red yeasts, from benthic animals and sediments collected from the deep-sea floor in various areas in the northwest Pacific Ocean. Comparing the yeast
Caldivirga maquilingensis gen. nov., sp. nov., a new genus of rod-shaped crenarchaeote isolated from a hot spring in the Philippines.
Two novel hyperthermophilic, rod-shaped crenarchaeotes were isolated from an acidic hot spring in the Philippines and it was revealed that they represented an independent lineage in the family Thermoproteaceae.
Phylogenetic and phenotypic evidence for the transfer of Eubacterium aerofaciens to the genus Collinsella as Collinsella aerofaciens gen. nov., comb. nov.
Based on a 16S rRNA sequence divergence of greater than 9% with Coriobacterium glomerans and the presence of a unique peptidoglycan type, a new genus, Collinsella, is proposed for Eubacterium aerofaciens, with one species, Coll insella aer ofaciens.