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A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications
The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has provided a myriad of applications for biological systems. Over the last several years, mutagenesis studies have improvedExpand
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Rapid and persistent modulation of actin dynamics regulates postsynaptic reorganization underlying bidirectional plasticity
The synapse is a highly organized cellular specialization whose structure and composition are reorganized, both positively and negatively, depending on the strength of input signals. The mechanismsExpand
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An Expanded Palette of Genetically Encoded Ca2+ Indicators
Directed protein evolution provides a series of fluorescent protein-based indicators for multicolor Ca2+ imaging. Engineered fluorescent protein (FP) chimeras that modulate their fluorescence inExpand
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Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators
Adenosine 5′-triphosphate (ATP) is the major energy currency of cells and is involved in many cellular processes. However, there is no method for real-time monitoring of ATP levels inside individualExpand
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Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins.
Fluorescence resonance energy transfer (FRET) technology has been used to develop genetically encoded fluorescent indicators for various cellular functions. Although most indicators have cyan- andExpand
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Circularly permuted green fluorescent proteins engineered to sense Ca2+
To visualize Ca2+-dependent protein–protein interactions in living cells by fluorescence readouts, we used a circularly permuted green fluorescent protein (cpGFP), in which the amino and carboxylExpand
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Spatio-temporal images of growth-factor-induced activation of Ras and Rap1
G proteins of the Ras family function as molecular switches in many signalling cascades; however, little is known about where they become activated in living cells. Here we use FRET (fluorescentExpand
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Spatio-temporal activation of caspase revealed by indicator that is insensitive to environmental effects
Indicator molecules for caspase-3 activation have been reported that use fluorescence resonance energy transfer (FRET) between an enhanced cyan fluorescent protein (the donor) and enhanced yellowExpand
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Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer.
GFP (green fluorescent protein)-based FRET (fluorescence resonance energy transfer) technology has facilitated the exploration of the spatio-temporal patterns of cellular signalling. While mostExpand
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Crystal Structure of Venus, a Yellow Fluorescent Protein with Improved Maturation and Reduced Environmental Sensitivity*
Yellow emission variants of green fluorescent protein (GFP) have been found useful in a variety of applications in biological systems due to their red-shifted emission spectrum and sensitivity toExpand
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