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A revised medium for rapid growth and bio assays with tobacco tissue cultures
In experiments with tobacco tissue cultured on White's modified medium supplemenk'd with kiticthi and hidoleacctic acid, a slrikin^' fourlo (ive-told intTease iu yield was ohtaitu-d within a three to Tour week j^rowth period on addition of an aqtteotis exlrarl of tobacco leaves (Fi^'ures 1 and 2). Expand
Plant Propagation Through Tissue Cultures
The development of a strategy for the establishment of aseptic culture and its application in the context of a desert environment. Expand
Plant tissue culture media
The development of the various types of tissue culture has been based on empirical approaches, and some of the observations recorded in the literature may not be typical for plant cells. Expand
Evaluation of parameters in the isolation of viable protoplasts from cultured tobacco cells.
The procedure enabled 30% of the tobacco cells to form protoplasts, 80% of which regenerated cell walls in 4 days and 40% resumed cell division activity when returned to cell culture medium. Expand
Somatic embryogenesis in avocado (Persea americana Mill.) in vitro
A tissue culture procedure was developed for the regeneration of somatic embryos from callus cultures of the avocado, Persea americana, and attempts to induce a higher incidence of germination were unsuccessful. Expand
Rejuvenation of Sequoia sempervirens by Repeated Grafting of Shoot Tips onto Juvenile Rootstocks in Vitro: Model for Phase Reversal of Trees.
Repeated grafting of 1.5-centimeter long shoot tips from an adult Sequoia sempervirens tree onto fresh, rooted juvenile stem cuttings in vitro resulted in progressive restoration of juvenile traits.Expand