New mammalian expression vectors
- B. Moss, O. Elroy-Stein, T. Mizukami, W. Alexander, T. Fuerst
- BiologyNature
- 1 November 1990
The RNA polymerases from T7 and related bacteriophages, in conjunction with elements of DNA and RNA viruses, can be used in novel ways for expression of genes in mammalian cells.
Identification of cell-active lysine specific demethylase 1-selective inhibitors.
- Rie Ueda, Takayoshi Suzuki, N. Miyata
- Biology, ChemistryJournal of the American Chemical Society
- 16 November 2009
The first small-molecule LSD1-selective inhibitors are identified and show in vivo H3K4-methylating activity and antiproliferative activity and should be useful as lead structures for anticancer drugs and as tools for studying the biological roles of LSD1.
Identification of SAP155 as the target of GEX1A (Herboxidiene), an antitumor natural product.
- M. Hasegawa, Tatsuhiro Miura, T. Mizukami
- Biology, ChemistryACS Chemical Biology
- 13 January 2011
GEX1A serves as a novel splicing inhibitor that specifically impairs the SF3b function by binding to SAP155 protein, a subunit ofSF3b responsible for pre-mRNA splicing.
G1 phase accumulation induced by UCN-01 is associated with dephosphorylation of Rb and CDK2 proteins as well as induction of CDK inhibitor p21/Cip1/WAF1/Sdi1 in p53-mutated human epidermoid carcinoma…
- T. Akiyama, T. Yoshida, S. Akinaga
- Biology, ChemistryCancer Research
- 15 April 1997
Results suggest that G1-phase accumulation induced by UCN-01 is associated with dephosphorylation of Rb and CDK2 proteins as well as induction of CDK inhibitors p21 and p27.
Design, synthesis, enzyme-inhibitory activity, and effect on human cancer cells of a novel series of jumonji domain-containing protein 2 histone demethylase inhibitors.
- Shohei Hamada, Takayoshi Suzuki, N. Miyata
- Chemistry, BiologyJournal of Medicinal Chemistry
- 14 July 2010
Findings suggest that combination treatment with JMJD2 inhibitors and LSD1 inhibitors may represent a novel strategy for anticancer chemotherapy.
Product review. New mammalian expression vectors.
- B. Moss, O. Elroy-Stein, T. Mizukami, W. Alexander, T. Fuerst
- BiologyNature
- 1990
Cloning and expression of cDNA for salmon growth hormone in Escherichia coli.
- S. Sekine, T. Mizukami, H. Kawauchi
- BiologyProceedings of the National Academy of Sciences…
- 1 July 1985
Mature sGH was efficiently expressed in Escherichia coli carrying a plasmid in which the sGH cDNA was under control of the E. coli trp promoter; sGH comprised about 15% of the total cellular protein in such bacteria.
Selective killing of HIV-infected cells by recombinant human CD4-Pseudomonas exotoxin hybrid protein
- V. Chaudhary, T. Mizukami, E. Berger
- BiologyNature
- 22 September 1988
The purification and characterization of a recombinant protein produced in Escherichia coli that contains the HIV-binding portion of the human CD4 molecule linked to active regions of Pseudomonas exotoxin A is described, which represents a promising novel therapeutic agent for the treatment of AIDS.
Rapid discovery of highly potent and selective inhibitors of histone deacetylase 8 using click chemistry to generate candidate libraries.
- Takayoshi Suzuki, Yosuke Ota, N. Miyata
- Chemistry, BiologyJournal of Medicinal Chemistry
- 12 November 2012
A library of HDAC8-selective inhibitors candidates, each containing a zinc-binding group that coordinates with the active-site zinc ion, linked via a triazole moiety to a capping structure that interacts with residues on the rim of the active site were designed.
GEX1 compounds, novel antitumor antibiotics related to herboxidiene, produced by Streptomyces sp. I. Taxonomy, production, isolation, physicochemical properties and biological activities.
- Y. Sakai, Tetsuo Yoshida, T. Mizukami
- Chemistry, BiologyJournal of antibiotics (Tokyo. )
- 1 October 2002
Six structurally related antitumor antibiotics named GEX1 compounds were isolated from a culture broth of Streptomyces sp. GEX1A was identified as a known herbicide, herboxidiene, structurally…
...
...