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Protein phosphatase type 2B (calcineurin)‐mediated, FK506‐sensitive regulation of intracellular ions in yeast is an important determinant for adaptation to high salt stress conditions.
The growth behaviour and intracellular Na+ and K+ of wild type cells in high NaCl medium became very similar to those of the PP2B‐deficient mutant in a manner dependent on the presence of the FK506 binding protein. Expand
Isolation and characterization of SSE1 and SSE2, new members of the yeast HSP70 multigene family.
Two new members of the Saccharomyces cerevisiae heat-shock protein 70 multigene (HSP70) family were isolated from a yeast expression library using antisera made against a yeast calmodulin-binding fraction, and their predicted amino acid sequences are highly homologous to each other. Expand
Effect of Ethanol on Cell Growth of Budding Yeast: Genes That Are Important for Cell Growth in the Presence of Ethanol
It is found that the tyrosine kinase Swe1, the negative regulator of Cdc28-Clb kinase, is related to the regulation of cell growth in the presence of ethanol, and that a signal produced by ethanol causes a transient up-regulation of Swe1. Expand
A mutation in NPS1/STH1, an essential gene encoding a component of a novel chromatin-remodeling complex RSC, alters the chromatin structure of Saccharomyces cerevisiae centromeres
In the nps1-105 mutant, impaired chromatin structure surrounding centromeres may lead to an impairment of kinetochore function and the cells arrest at G2/M phase through the spindle-assembly checkpoint system. Expand
GSK‐3 kinase Mck1 and calcineurin coordinately mediate Hsl1 down‐regulation by Ca2+ in budding yeast
The genetic screening identified the MCK1 gene, which encodes a glycogen synthetase kinase‐3 family protein kinase, as a component of the Ca2+ signaling pathway and indicated that Mck1 functions downstream of the Mpk1 pathway and down‐regulates Hsl1, an inhibitory kinase of Swe1. Expand
Role of calcineurin and Mpk1 in regulating the onset of mitosis in budding yeast
Calcineurin and Mpk1 regulate Swe1 activation at the transcriptional and post-translational levels, respectively, and both are required for the calcium-induced delay in G2 phase, which induce a G2-phase delay in response to hypotonic shock. Expand
RRS1, a Conserved Essential Gene, Encodes a Novel Regulatory Protein Required for Ribosome Biogenesis inSaccharomyces cerevisiae
A cold-sensitive mutant with a mutation in a gene termed RRS1 (regulator of ribosome synthesis), which appeared to be defective in the signaling pathway, was isolated and greatly reduced transcriptional repression of both rRNA and ribosomal protein genes that is caused by a secretory defect. Expand
PKC1, a protein kinase C homologue of Saccharomyces cerevisiae, participates in microtubule function through the yeast EB1 homologue, BIM1
Background RSC is a chromatin‐remodelling complex of Saccharomyces cerevisiae and essential for growth. Its catalytic subunit is encoded by the NPS1/STH1 gene. At the present time, little is knownExpand
Saccharomyces cerevisiae YDR1, which encodes a member of the ATP-binding cassette (ABC) superfamily, is required for multidrug resistance
The transcription of YDR1 and SNQ2 was induced by various drugs, both relevant and irrelevant to the resistance caused by the gene, suggesting that drug specificity can be mainly attributed to the functional difference of the putative transporters. Expand
Yeast gene YRR1, which is required for resistance to 4‐nitroquinoline N‐oxide, mediates transcriptional activation of the multidrug resistance transporter gene SNQ2
It is established that Yrr1p is important for 4‐NQO resistance by mediating transcriptional activation of the SNQ2 gene in response to the stress imposed by 4‐ NQO. Expand