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Chemical inhibitor of nonapoptotic cell death with therapeutic potential for ischemic brain injury
The mechanism of apoptosis has been extensively characterized over the past decade, but little is known about alternative forms of regulated cell death. Although stimulation of the Fas/TNFR receptorExpand
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Microtubule polymerization dynamics.
The polymerization dynamics of microtubules are central to their biological functions. Polymerization dynamics allow microtubules to adopt spatial arrangements that can change rapidly in response toExpand
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Dynamic instability of microtubule growth
We report here that microtubules in vitro coexist in growing and shrinking populations which interconvert rather infrequently. This dynamic instability is a general property of microtubules and mayExpand
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A chemical method for fast and sensitive detection of DNA synthesis in vivo
  • A. Salic, T. Mitchison
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences
  • 19 February 2008
We have developed a method to detect DNA synthesis in proliferating cells, based on the incorporation of 5-ethynyl-2′-deoxyuridine (EdU) and its subsequent detection by a fluorescent azide through aExpand
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Self- and actin-templated assembly of Mammalian septins.
Septins are polymerizing GTPases required for cytokinesis and cortical organization. The principles by which they are targeted to, and assemble at, specific cell regions are unknown. We show thatExpand
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Dissecting Temporal and Spatial Control of Cytokinesis with a Myosin II Inhibitor
Completion of cell division during cytokinesis requires temporally and spatially regulated communication from the microtubule cytoskeleton to the actin cytoskeleton and the cell membrane. WeExpand
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Kin I Kinesins Are Microtubule-Destabilizing Enzymes
Using in vitro assays with purified proteins, we show that XKCM1 and XKIF2, two distinct members of the internal catalytic domain (Kin I) kinesin subfamily, catalytically destabilize microtubulesExpand
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Reassembly of contractile actin cortex in cell blebs
Contractile actin cortex is involved in cell morphogenesis, movement, and cytokinesis, but its organization and assembly are poorly understood. During blebbing, the membrane detaches from the cortexExpand
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A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish
Barrier structures (for example, epithelia around tissues and plasma membranes around cells) are required for internal homeostasis and protection from pathogens. Wound detection and healing representExpand
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Probing Spindle Assembly Mechanisms with Monastrol, a Small Molecule Inhibitor of the Mitotic Kinesin, Eg5
Monastrol, a cell-permeable small molecule inhibitor of the mitotic kinesin, Eg5, arrests cells in mitosis with monoastral spindles. Here, we use monastrol to probe mitotic mechanisms. We find thatExpand
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