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Tetracycline-Regulatable System To Tightly Control Gene Expression in the Pathogenic Fungus Candida albicans
ABSTRACT Conventional tools for elucidating gene function are relatively scarce in Candida albicans, the most prevalent human fungal pathogen. To this end, we developed a convenient system to controlExpand
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Roles of three histidine kinase genes in hyphal development and virulence of the pathogenic fungus Candida albicans.
The pathogenic fungus Candida albicans harbors three histidine kinase genes called CaSLN1, CaNIK1, and CaHK1. The disruption of any one of these three genes impaired the hyphal formation andExpand
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Characterization and gene cloning of 1,3-beta-D-glucan synthase from Saccharomyces cerevisiae.
1,3-beta-D-Glucan synthase of Saccharomyces cerevisiae was solubilized and purified up to 700-fold by product entrapment. The specific activity of the partially purified enzyme was around 4 mumolExpand
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Saccharomyces cerevisiae GNA1, an Essential Gene Encoding a Novel Acetyltransferase Involved in UDP-N-acetylglucosamine Synthesis*
The Saccharomyces cerevisiae gene,YFL017C, for a putative acetyltransferase was characterized. Disruption of YFL017C was lethal, leading to a morphology similar to those caused by the depletionExpand
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Isolation of CaSLN1 and CaNIK1, the genes for osmosensing histidine kinase homologues, from the pathogenic fungus Candida albicans.
Recent studies have revealed that fungi possess a mechanism similar to bacterial two-component systems to respond to extracellular changes in osmolarity. In Saccharomyces cerevisiae, Sln1p containsExpand
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Characterization of Chitin Synthase 2 of Saccharomyces cerevisiae.
Chitin synthase 2 of Saccharomyces cerevisiae was characterized by means of site-directed mutagenesis and subsequent expression of the mutant enzymes in yeast cells. Chitin synthase 2 shares a regionExpand
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The Eukaryotic UDP-N-Acetylglucosamine Pyrophosphorylases
A search of the yeast data base for a protein homologous to Escherichia coliUDP-N-acetylglucosamine pyrophosphorylase yieldedUAP1 (UDP-N-acetylglucosaminepyrophosphorylase), the SaccharomycesExpand
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Cloning of the Candida albicans homolog of Saccharomyces cerevisiae GSC1/FKS1 and its involvement in beta-1,3-glucan synthesis.
Saccharomyces cerevisiae GSC1 (also called FKS1) and GSC2 (also called FKS2) have been identified as the genes for putative catalytic subunits of beta-1,3-glucan synthase. We have cloned threeExpand
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Role of three chitin synthase genes in the growth of Candida albicans.
The CHS2 and CHS3 genes of Candida albicans were disrupted. The double disruptant was still viable. Assessment of chitin and of calcofluor white resistance shows that CHS1 is responsible for septumExpand
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Identification and characterization of the genes for N-acetylglucosamine kinase and N-acetylglucosamine-phosphate deacetylase in the pathogenic fungus Candida albicans.
Like bacteria and many fungi, the pathogenic fungus Candida albicans can utilize GlcNAc as a carbon source for growth. A cluster of six genes was identified in the C. albicans genome. One of theExpand
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