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Initial sequencing and analysis of the human genome.
The results of an international collaboration to produce and make freely available a draft sequence of the human genome are reported and an initial analysis is presented, describing some of the insights that can be gleaned from the sequence.
Genome-wide maps of chromatin state in pluripotent and lineage-committed cells
The application of single-molecule-based sequencing technology for high-throughput profiling of histone modifications in mammalian cells is reported and it is shown that chromatin state can be read in an allele-specific manner by using single nucleotide polymorphisms.
A Bivalent Chromatin Structure Marks Key Developmental Genes in Embryonic Stem Cells
Massively parallel digital transcriptional profiling of single cells
A droplet-based system that enables 3′ mRNA counting of up to tens of thousands of single cells per sample is described and enables characterization of diverse biological systems with single cell mRNA analysis.
The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells
Monocle is described, an unsupervised algorithm that increases the temporal resolution of transcriptome dynamics using single-cell RNA-Seq data collected at multiple time points that revealed switch-like changes in expression of key regulatory factors, sequential waves of gene regulation, and expression of regulators that were not known to act in differentiation.
Genome-scale DNA methylation maps of pluripotent and differentiated cells
Low-throughput reduced representation bisulphite sequencing is established as a powerful technology for epigenetic profiling of cell populations relevant to developmental biology, cancer and regenerative medicine.
Chromatin signature reveals over a thousand highly conserved large non-coding RNAs in mammals
It is demonstrated that specific lincRNAs are transcriptionally regulated by key transcription factors in these processes such as p53, NFκB, Sox2, Oct4 (also known as Pou5f1) and Nanog, defining a unique collection of functional linc RNAs that are highly conserved and implicated in diverse biological processes.
Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells
This work shows that lentiviral delivery of a genome-scale CRISPR-Cas9 knockout (GeCKO) library targeting 18,080 genes with 64,751 unique guide sequences enables both negative and positive selection screening in human cells, and observes a high level of consistency between independent guide RNAs targeting the same gene and a high rate of hit confirmation.
Genome sequence, comparative analysis and haplotype structure of the domestic dog
A high-quality draft genome sequence of the domestic dog is reported, together with a dense map of single nucleotide polymorphisms (SNPs) across breeds, to shed light on the structure and evolution of genomes and genes.
The NIH Roadmap Epigenomics Mapping Consortium
The NIH Roadmap Epigenomics Mapping Consortium aims to produce a public resource of epigenomic maps for stem cells and primary ex vivo tissues selected to represent the normal counterparts of tissues…