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Characterization of hen egg white- and yolk-riboflavin binding proteins and amino acid sequence of egg white-riboflavin binding protein.
The amino acid sequences around phosphoserines showed that phosphorylation might occur at a serine residue in one of the following sequences; Ser-X-Glu or Ser- X-Ser(P).
Quantitation and isomeric structure analysis of free oligosaccharides present in the cytosol fraction of mouse liver: detection of a free disialobiantennary oligosaccharide and glucosylated
Degradation by these enzymes seems to be the main pathway by which oligomannosides are degraded in mouse cytosol, suggesting that cytosolic endo-beta-N-acetylglucosaminidase and neutral alpha-mannosidase participate in the degradation, because these enzymes have suitable substrate specificities for the production of M5B'.
Purification and characterization of chitinase produced by Streptomyces erythraeus.
The results showed that the binding mode of SE chitinase to the substrate is similar to that of HEW and SE lysozymes, and could hydrolyze chit in and its derivatives, but could not hydrolyzes cell walls of Micrococcus lysodeikticus.
Purification and characterization of endo-beta-N-acetylglucosaminidase from hen oviduct.
Substrate specificity results using pyridylaminated N-linked sugar chains revealed that Endo-HO hydrolyzed oligomannose-type sugar chains faster than complex- and hybrid-type chains, and that sugar chains containing the Man alpha1-2Manalpha1-3Man beta1-4GlcNAcbeta1-GlcN Ac structure were good substrates for the enzyme.
Glucose Trimming of N-Glycan in Endoplasmic Reticulum Is Indispensable for the Growth of Raphanus sativus Seedling (kaiware radish)
  • T. Mega
  • Biology
    Bioscience, biotechnology, and biochemistry
  • 1 January 2005
The results suggest that N-glycan processing after glucosidase processing is dispensable for plant growth and cell differentiation.
Conversion of the carbohydrate structures of glycoproteins in roots of Raphanus sativus using several glycosidase inhibitors.
  • T. Mega
  • Biology, Chemistry
    Journal of biochemistry
  • 1 October 2004
The results show that the N-glycan processing in both the endoplasmic reticulum (ER) and Golgi apparatus can be controlled artificially using glycosidase inhibitor, and that the glucosidase inhibitors could be useful for the study of the function of N- glycans in plants.
Comparison of the amino acid sequences of hen plasma-, yolk-, and white-riboflavin binding proteins.
The results led to the conclusion that yolk- and white-RBPs are bio-synthesized from the same gene in the different organs (liver and oviduct).
Studies on the oligosaccharide chains of human alpha 1-protease inhibitor. I. Isolation of glycopeptides.
The carbohydrate and amino acid compositions of the glycopeptides obtained by these treatments demonstrated that oligosaccharide side chains are attached to three asparaginyl residues which are located in separate regions of the polypeptide chain, and that the carbohydrate chains are made of two different carbohydrate compositions.
Determination of lectin-sugar binding constants by microequilibrium dialysis coupled with high performance liquid chromatography.
  • T. Mega, S. Hase
  • Chemistry, Biology
    Journal of biochemistry
  • 1 April 1991
High performance liquid chromatography was used to determine the concentrations of free ligands after equilibrium dialysis to examine lectin-sugar interactions and determined the binding constant, Ka, and the concentration of total binding sites, [L]t, from the trace amounts of sugars and lectins.
Characterization of carbohydrate-binding specificity of concanavalin A by competitive binding of pyridylamino sugar chains.
Carbohydrate-binding specificity of Con A was characterized by competitive binding studies of pyridylamino (PA) sugar chains. PA-derivatives of 17 oligomannose-type sugar chains, Man1-9GlcNAc2-PA,