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Structure and chemistry of cytochrome P450.
TLDR
This review will concentrate on findings with P-450cam of the Pseudomonas putida camphor-5-exo-hydroxylase, and attention will be drawn to parallel and contrasting examples from other P- 450s as appropriate. Expand
Hydroxylation of camphor by reduced oxy-cytochrome P450cam: mechanistic implications of EPR and ENDOR studies of catalytic intermediates in native and mutant enzymes.
TLDR
Chemical and EPR quantitations indicate the reaction pathway involving 5B yields 5-exo-hydroxycamphor quantitatively, and gamma-irradiation at cryogenic temperatures of the ternary complexes of camphor, dioxygen, and ferro-cytochrome P450cam uses EPR and ENDOR spectroscopies to characterize the primary product of reduction as well as subsequent states created by annealing reduced oxyP450. Expand
Thirty years of microbial P450 monooxygenase research: peroxo-heme intermediates--the central bus station in heme oxygenase catalysis.
TLDR
This volume provides an opportunity to recognize the breakthroughs of the "Hayaishi School" and has been the prolific contributions of Professor Ishimura et al. Expand
Resonance Raman characterization of the peroxo and hydroperoxo intermediates in cytochrome P450.
TLDR
Experimental Resonance Raman studies of intermediates generated by cryoreduction of the oxyferrous complex of the D251N mutant of cytochrome P450(cam) (CYP101) are reported, showing that the nu(O-O) mode decreases from a superoxo-like frequency near approximately 1130 cm(-1) to 792 cm (-1) upon reduction. Expand
Cytochrome p450 compound I.
TLDR
Formation of the Compound I derivative of a P450 enzyme is reported by laser flash photolysis oxidation of the corresponding Compound II species, an iron(IV)-oxo neutral porphyrin intermediate. Expand
MECHANISTIC ENZYMOLOGY OF OXYGEN ACTIVATION BY THE CYTOCHROMES P450
TLDR
The detailed mechanism of P450 dioxygen scission is discussed utilizing the CYP101 hydroxylation of camphor as a model system and a structural and spectroscopic analysis of the nature of critical intermediate states in the reaction is discussed. Expand
Structural and Molecular Characterization of Iron-sensing Hemerythrin-like Domain within F-box and Leucine-rich Repeat Protein 5 (FBXL5)♦
TLDR
The atomic structure of the FBXL5 N terminus is presented, a hemerythrin-like α-helical bundle fold not previously observed in mammalian proteins, which provides insights into the mechanisms by whichFBXL5 serves as a unique mammalian metabolic sensor. Expand
Cyanobacterial aldehyde deformylase oxygenation of aldehydes yields n-1 aldehydes and alcohols in addition to alkanes.
TLDR
It is shown that ADO also catalyzes incorporation of an oxygen atom from O2 into the alkane product to yield alcohol and aldehyde products, and the nature of the diiron cluster and the newly recognized products from ADO catalysis hold implications for the mechanism of C-C bond cleavage. Expand
Mixed regiospecificity compromises alkene synthesis by a cytochrome P450 peroxygenase from Methylobacterium populi.
TLDR
It is shown that a second CYP152, CYP-MP from Methylobacterium populi ATCC BAA 705, also catalyzes oxidative substrate decarboxylation, suggesting an importance of substrate coordination for regulating alkene production. Expand
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