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Specific binding of HIV-1 recombinant Rev protein to the Rev-responsive element in vitro
It is demonstrated that Rev protein, purified from Escherichia coli, binds in a sequence-specific manner to the RRE element in vitro, supporting the hypothesis that Rev interacts directly with the Rre element. Expand
Inhibition of angiogenesis by recombinant human platelet factor-4 and related peptides.
Recombinant human platelet factor-4 (rhPF4), purified from Escherichia coli, inhibited blood vessel proliferation in the chicken chorioallantoic membrane in a dose-dependent manner and suggested that the angiostatic effect was due to specific inhibition of growth factor-stimulated endothelial cell proliferation. Expand
Platelet Factor-4 Inhibits the Mitogenic Activity of VEGF121 and VEGF165 Using Several Concurrent Mechanisms (*)
PF4 can bind to heparin binding proteins such as VEGF165 leading to an inhibition of their receptor binding ability, and it is observed that PF4 inhibits efficiently the V EGF165 induced proliferation of vascular endothelial cells. Expand
Inhibition of tumor growth in mice by an analogue of platelet factor 4 that lacks affinity for heparin and retains potent angiostatic activity.
The present findings support the conclusion that both r PF4 and rPF4-241 inhibit tumor growth by suppression of tumor-induced neovascularization and suggest that PF4 may play a more specific role in modulation of blood vessel development than previously recognized. Expand
Crystal structure of recombinant human platelet factor 4.
The crystal structure of human platelet factor 4 (PF4) has been solved to a resolution of 2.4 A by molecular replacement and refined to an R-factor of 24.1%. The structure consists of fourExpand
Heparin binding to platelet factor-4. An NMR and site-directed mutagenesis study: arginine residues are crucial for binding.
NMR data presented here indicate that heparin binding to native platelet factor-4-M2, while not perturbing the overall structure of the protein, does perturb specific side-chain proton resonances which map to spatially related residues within a ring of positively charged side chains on the surface of tetrameric PF4- M2. Expand
Growth inhibition of murine melanoma and human colon carcinoma by recombinant human platelet factor 4.
The observed antitumor effects of rHuPF 4 might be due to the inhibition of angiogenesis, which could have implications for the development of novel therapeutic approaches to angiogenic diseases. Expand
Inhibition of development of murine melanoma lung metastases by systemic administration of recombinant platelet factor 4.
Intravenous administration of rPF4 significantly inhibited the development of metastatic lung nodules in a dose-dependent fashion as assessed by both lesion number and lung weight, and these findings are consistent with the known angiostatic properties of r PF4. Expand
Reversal of heparin anticoagulation by recombinant platelet factor 4 and protamine sulfate in baboons during cardiopulmonary bypass.
It is concluded that recombinant platelet factor 4 is effective and safe in baboons, does not have an anticoagulant effect with excess concentration, and reverses in vivo heparin more rapidly than protamine. Expand
Circular dichroism studies of the HIV-1 Rev protein and its specific RNA binding site.
The circular dichroism (CD) spectrum of the Rev protein from HIV-1 indicates that Rev contains about 50% alpha helix and 25% beta sheet at 5 degrees C in potassium phosphate buffer, pH 3, and 300 mMExpand