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Bicarbonate conductance and pH regulatory capability of cystic fibrosis transmembrane conductance regulator.
The cystic fibrosis transmembrane conductance regulator (CFTR) is an epithelial Cl- channel regulated by protein kinase A. The most common mutation in cystic fibrosis (CF), deletion of Phe-508 (deltaExpand
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Mechanisms of pH Regulation in the Regulated Secretory Pathway*
A precise pH gradient between organelles of the regulated secretory pathway is required for sorting and processing of prohormones. We studied pH regulation in live endocrine cells by targetingExpand
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Intracellular ion activities and membrane transport in parietal cells measured with fluorescent dyes.
Publisher Summary This chapter discusses the methods used for measuring intracellular pH, [Cai2+] (Cai2+, with Fura-2) and [Na +] in single, identified cells of gastric glands isolated from rabbits.Expand
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Organelle pH studies using targeted avidin and fluorescein-biotin.
BACKGROUND Mammalian organelles of the secretory pathway are of differing pH. The pH values form a decreasing gradient: the endoplasmic reticulum (ER) is nearly neutral, the Golgi is mildly acidicExpand
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Expression and Characterization of a Redox-Sensing Green Fluorescent Protein (Reduction-Oxidation-Sensitive Green Fluorescent Protein) in Arabidopsis
Arabidopsis (Arabidopsis thaliana) was transformed with a redox-sensing green fluorescent protein (reduction-oxidation-sensitive green fluorescent protein [roGFP]), with expression targeted to eitherExpand
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cAMP-independent activation of CFTR Cl channels by the tyrosine kinase inhibitor genistein.
Genistein, a protein tyrosine kinase inhibitor, activates the cystic fibrosis transmembrane conductance regulator (CFTR) in transfected NIH-3T3 fibroblasts that express the CFTR (3T3-CFTR). CFTRExpand
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CFTR displays voltage dependence and two gating modes during stimulation
The patch-clamp technique in conjunction with current noise analysis was employed to clarify the events underlying the regulation of the CFTR (cystic fibrosis transmembrane conductance regulator)Expand
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Innate immune response in CF airway epithelia: hyperinflammatory?
  • T. Machen
  • Medicine, Biology
  • American journal of physiology. Cell physiology
  • 1 August 2006
The lack of functional cystic fibrosis (CF) transmembrane conductance regulator (CFTR) in the apical membranes of CF airway epithelial cells abolishes cAMP-stimulated anion transport, and bacteria,Expand
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NADPH Oxidase-dependent Acid Production in Airway Epithelial Cells*
The purpose of this study was to determine the role of NADPH oxidase in H+ secretion by airway epithelia. In whole cell patch clamp recordings primary human tracheal epithelial cells (hTE) and theExpand
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Cystic fibrosis airway epithelial cell polarity and bacterial flagellin determine host response to Pseudomonas aeruginosa
The role of epithelial polarity and bacterial factors in the control of the innate immune response of airway epithelial cells to Pseudomonas aeruginosa PAK was investigated using a human, nasalExpand
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