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Imaging Dynamic Redox Changes in Mammalian Cells with Green Fluorescent Protein Indicators*
- C. Dooley, T. M. Dore, G. Hanson, W. Jackson, S. Remington, R. Tsien
- BiologyJournal of Biological Chemistry
- 21 May 2004
The response of the redox probes under physiological redox changes during superoxide bursts in macrophage cells, hyperoxic and hypoxic conditions, and in responses to H2O2-stimulating agents, e.g. epidermal growth factor and lysophosphatidic acid is investigated.
Brominated 7-hydroxycoumarin-4-ylmethyls: photolabile protecting groups with biologically useful cross-sections for two photon photolysis.
- T. Furuta, S. S. Wang, R. Tsien
- Chemistry, BiologyProceedings of the National Academy of Sciences…
- 16 February 1999
It is shown that brominated 7-hydroxycoumarin-4-ylmethyl esters and carbamates efficiently release carboxylates and amines on photolysis, with one- and two-photon cross sections up to one or two orders of magnitude better than previously available.
Brominated hydroxyquinoline as a photolabile protecting group with sensitivity to multiphoton excitation.
The synthesis and photochemistry of a new photolabile protecting group for carboxylic acids based on 8-bromo-7-hydroxyquinoline is described, which has sufficient sensitivity to multiphoton-induced photolysis for use in vivo and increased solubility and low fluorescence make it quite useful as a caging group for biological messengers.
Inhibition of the CaaX proteases Rce1p and Ste24p by peptidyl (acyloxy)methyl ketones.
Targeting Type 2 Diabetes with C-Glucosyl Dihydrochalcones as Selective Sodium Glucose Co-Transporter 2 (SGLT2) Inhibitors: Synthesis and Biological Evaluation.
- Ana R. Jesus, Diogo Vila-Viçosa, M. Machuqueiro, A. P. Marques, T. M. Dore, A. Rauter
- Chemistry, BiologyJournal of medicinal chemistry
- 18 January 2017
The results point toward the discovery of structures that are potent and highly selective inhibitors of SGLT2, and the interaction of a C-glucosyl dihydrochalcone with a POPC membrane was modeled computationally, providing evidence that it is not a pan-assay interference compound.
Bhc‐cNMPs as either Water‐Soluble or Membrane‐Permeant Photoreleasable Cyclic Nucleotides for both One‐ and Two‐Photon Excitation
- T. Furuta, H. Takeuchi, R. Tsien
- Biology, ChemistryChembiochem : a European journal of chemical…
- 6 August 2004
The synthesis, photochemistry, and biological testing of 6‐bromo‐7‐hydroxycoumarin‐4‐ylmethyl esters of cNMP and their acetyl derivatives (Bhc‐cNMP/Ac) as new caged second messengers showed that Bhc and Bhc/Ac caged compounds have great potential for use in many cell biological applications.
Inhibition of kinesin motor proteins by adociasulfate-2.
The data suggest that AS-2 is not a classical 1:1 inhibitor, but a rodlike aggregate that mimics microtubules is complexed with kinesin and inhibits its ATPase activity, suggesting aggregates of a chiral natural product can have interesting and biologically relevant properties.
Rce1: mechanism and inhibition
- S. Hampton, T. M. Dore, W. K. Schmidt
- Biology, ChemistryCritical reviews in biochemistry and molecular…
- 9 February 2018
Small-molecule inhibitor discovery is currently limited by the lack of structural information on a eukaryotic enzyme, but a set of 8-hydroxyquinoline derivatives has demonstrated an ability to mislocalize all three mammalian Ras isoforms, giving optimism that potent, selective inhibitors might be developed.
Multiphoton Phototriggers for Exploring Cell Physiology
- T. M. Dore
- 27 October 2005
8-Bromo-7-hydroxyquinoline as a photoremovable protecting group for physiological use: mechanism and scope.
- Yue Zhu, Christopher M. Pavlos, J. Toscano, T. M. Dore
- BiologyJournal of the American Chemical Society
- 15 March 2006
Two-photon excitation (2PE) of "caged" biomolecules represents a powerful method to investigate the temporal and spatial relevance of physiological function in real time and on living tissue, because…