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Importing Mitochondrial Proteins: Machineries and Mechanisms
TLDR
Recent insights into the importing and sorting of mitochondrial proteins and their contributions to mitochondrial biogenesis are discussed. Expand
The J‐protein family: modulating protein assembly, disassembly and translocation
TLDR
It is suggested that J‐like proteins might function to regulate the activity of bona fide J proteins during protein translocation, assembly and disassembly. Expand
Evolution of the Molecular Machines for Protein Import into Mitochondria
TLDR
In creating mitochondria some 2 billion years ago, the first eukaryotes needed to establish protein import machinery in the membranes of what was a bacterial endosymbiont, but the protein translocases that drive protein import into mitochondria have no obvious counterparts in bacteria, making it likely that these machines were created de novo. Expand
Structural insight into the biogenesis of β-barrel membrane proteins
TLDR
The structure of BamA is described, the central and essential component of the BAM complex, from two species of bacteria: Neisseria gonorrhoeae and Haemophilus ducreyi, which consists of a large periplasmic domain attached to a 16-strand transmembrane β-barrel domain. Expand
The Omp85 family of proteins is essential for outer membrane biogenesis in mitochondria and bacteria
TLDR
Members of the Omp85 protein family are found in eukaryotes ranging from plants to humans and are essential for cell viability in yeast and conditional omp85 mutants have defects that arise from compromised insertion of integral proteins. Expand
A protein complex containing Tho2, Hpr1, Mft1 and a novel protein, Thp2, connects transcription elongation with mitotic recombination in Saccharomyces cerevisiae
TLDR
An oligomeric complex containing Tho2, Hpr1, Mft1 and a novel protein that is mainly nuclear is isolated and it is proposed that this protein complex acts as a functional unit connecting transcription elongation with the incidence of mitotic recombination. Expand
Bipartite Signals Mediate Subcellular Targeting of Tail-anchored Membrane Proteins in Saccharomyces cerevisiae *
TLDR
Sequence analysis identified 55 tail-anchored proteins in Saccharomyces cerevisiae, with several novel proteins, including Prm3, which is found to be required for karyogamy and is tail- Anchored in the nuclear envelope. Expand
RAC, a stable ribosome-associated complex in yeast formed by the DnaK-DnaJ homologs Ssz1p and zuotin
TLDR
This work has identified the DnaK homolog Ssz1p/Pdr13p as zuotin's partner chaperone, and confirmed the existence of a functional complex RAC, which stimulates the translocation of a ribosome-bound mitochondrial precursor protein into mitochondria, providing evidence for its chaper one-like effect on nascent chains. Expand
The peripheral membrane subunits of the SAM complex function codependently in mitochondrial outer membrane biogenesis.
TLDR
By screening a library of sam35 mutants, it is shown that 13 distinct alleles were each specifically suppressed by overexpression of SAM37, and the role of Sam35 and Sam37 is discussed in terms of the problems peculiar to mitochondrial protein substrates. Expand
Discovery of an archetypal protein transport system in bacterial outer membranes
TLDR
The discovery of a new translocation and assembly module (TAM) that promotes efficient secretion of autotransporters in proteobacteria is discovered and provides a new target for the development of therapies to inhibit colonization by bacterial pathogens. Expand
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