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Pre‐mRNA processing enhancer (PPE) element increases the expression of an intronless thymidylate synthase gene but does not affect intron‐dependent S phase regulation
The goal of this study was to determine if the PPE element would overcome the dependence on introns for efficient expression and for S phase‐specific expression of transfected TS minigenes and found that insertion of the P PE element into an intronless TS minigene partially overcame intron dependence. Expand
Analysis of transcripts derived from sequences upstream of the bidirectional mouse thymidylate synthase promoter.
Analysis of the sequences of the cDNAs revealed that the upstream transcripts were not spliced at the potential 3' donor site but instead extended into a repetitive LINE sequence that begins 0.3 kb upstream of the TS promoter. Expand