T. L. Nedambale
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Developmental potential of vitrified holstein cattle embryos fertilized in vitro with sex-sorted sperm.
In vitro fertilization (IVF) is a feasible way to utilize sex-sorted sperm to produce offspring of a predetermined sex in the livestock industry. The objective of the present study was to examine the… Expand
Prolonging bovine sperm-oocyte incubation in modified medium 199 improves embryo development rate and the viability of vitrified blastocysts.
This study was conducted to compare the efficacy of four in vitro fertilization (IVF) media: Bracket and Oliphant's medium (BO), modified medium 199 (IVF-M199), modified Tyrode's medium (MTM), and… Expand
Comparison on in vitro fertilized bovine embryos cultured in KSOM or SOF and cryopreserved by slow freezing or vitrification.
- T. L. Nedambale, A. Dinnyés, W. Groen, J. Dobrinsky, X. Tian, X. Yang
- Medicine, Biology
- 1 August 2004
The objectives of this study were to identify an improved in vitro cell-free embryo culture system and to compare post-warming development of in vitro produced (IVP) bovine embryos following… Expand
Higher survival rate of vitrified and thawed in vitro produced bovine blastocysts following culture in defined medium supplemented with beta-mercaptoethanol.
The present study was conducted to compare bovine embryo developmental quality, after culture in different defined culture media, up to blastocyst stage, and subsequently cultured in media… Expand
Comparison of electro ejaculator and artificial vagina on semen collection from South African indigenous goat following assessment by Computer Aided Sperm Analysis.
South African indigenous bucks are raised under extensive conditions and are more likely to reject artificial vagina (AV) due to their limited contact with human beings, as they are less handled. The… Expand
Cryopreservation of South African indigenous goat semen
®V10 software. South African indigenous goats had total sperm cell motility rate of 83.1%, progressive sperm cell motility of 49.3% and non-progressive sperm cell motility of 33.9%. Moreover, acidic… Expand
Comparison of different extenders and storage temperature on the sperm motility characteristics of Kolbroek pig semen
Maintaining a successful pig artificial insemination programme depends on a number of factors, including evaluation of semen characteristics. This study compared the efficacy of different extenders… Expand
Improved development of ovine matured oocyte following solid surface vitrification (SSV): effect of cumulus cells and cytoskeleton stabilizer.
The objective of the present study was to examine the effects of cumulus cells, cytochalasin B (CB), and taxol on the development of ovine matured oocyte following solid surface vitrification (SSV).… Expand
Characterization of Sperm Cell Motility Rate of Southern African Indigenous Cockerel Semen following Analysis by Sperm Class Analyser
The Sperm Class Analyser (SCA) system (CASA) is advantageous and provides precise additional information that cannot be attained through the subjective manual sperm cell assessment methods. The aim… Expand
The characterisation and cryopreservation of Venda chicken semen
Abstract Objective To characterize Venda cocks semen, find a suitable short-term diluted semen storage temperature, find a suitable cryopreservation cryoprotectant and to investigate cryoprotectant… Expand