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RNA Splicing in the Chloroplast
The chloroplast genomes of Chlamydomonas spp. contain introns that belong to the two major classes found in organelles: group I and group II. Some of the members of both classes are ribozymes capableExpand
Revisiting with a Relative-Density Calibration Approach the Determination of Growth Rates of Microorganisms by Use of Optical Density Data from Liquid Cultures
This method was satisfactorily applied to study the growth of Escherichia coli and the cyanobacterium Anabaena spiroides. Expand
Optimization of DNA-directed immobilization on mixed oligo(ethylene glycol) monolayers for immunodetection.
The results indicated that a 50:1 ratio of oligo(ethylene glycol) (OEG)/COOH-terminated OEG and DNA sequences with 20mer are the best conditions found here for making a protein chip via a DNA-directed immobilization (DDI) method. Expand
Isothermal titration calorimetry for characterization of recombinant proteins.
The binding-linked reactions that strongly affect the observed thermodynamic parameters and must be dissected to obtain the intrinsic parameters that are necessary for the structure-based rational drug design are being demonstrated using inhibitors of Hsp90, an anticancer target protein. Expand
Phytochemical composition and larvicidal activity of essential oils from herbal plants
Main conclusionThe essential oils (EOs) of Plectranthus amboinicus showed the highest larvicidal activity among four herbal plants studied and β-caryophyllene might be the major component responsibleExpand
The down-regulation of galectin-1 expression is a specific biomarker of arsenic toxicity.
Galectin-1 gene knockdown mediates the apoptotic effects of arsenic in 3T3 and KB cells via regulation of the cellular arsenic levels, and it is proposed that down-regulation of GAL1 expression may be a useful and specific biomarker in assessing the toxicity of arsenic exposure. Expand
Extracellular metalloprotease gene of Streptomyces cacaoi: structure, nucleotide sequence and characterization of the cloned gene product.
The Npr protease contained putative zinc ligand-binding regions and two repeated motifs, Asp-Ser-Gly, similar to the active site residues of the aspartic acid and retroviral proteases, which led to the conclusion that it was processed from the 60-kDa pre-proenzyme form encoded by npr. Expand
Evolution and assembly of an extremely scrambled gene
The complex pattern of scrambling in the DNA polymerase α gene of Stylonychia lemnae is reported and direct repeats present at the boundaries between coding and noncoding sequences provide pointers to help guide assembly of the functional (macronuclear) gene. Expand
A kinetically efficient form of the Chlamydomonas self-splicing ribosomal RNA precursor.
  • T. Kuo, D. Herrin
  • Biology, Medicine
  • Biochemical and biophysical research…
  • 14 July 2000
The 23S rRNA gene of Chlamydomonas reinhardtii contains a group IA3 intron, Cr.LSU, whose splicing is essential for cell growth. To better understand Cr.LSU splicing, kinetic analyses were undertakenExpand
Characterization of the role of protein–cysteine residues in the binding with sodium arsenite
The current data suggest that the cysteine residues in GAL1 may play a critical role in the binding of arsenic, but that in the case of RCN3 and Prdx6, this interaction may be mediated by other factors. Expand