• Publications
  • Influence
Enzymatic Aspects of Isoprenoid Chain Elongation.
  • 289
  • 16
Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis. A key factor participating in natural rubber biosynthesis.
Natural rubber from Hevea brasiliensis is a high molecular mass polymer of isoprene units with cis-configuration. The enzyme responsible for the cis-1,4-polymerization of isoprene units has beenExpand
  • 134
  • 10
  • PDF
Crystal structure of cis-prenyl chain elongating enzyme, undecaprenyl diphosphate synthase
Undecaprenyl diphosphate synthase (UPS) catalyzes the cis-prenyl chain elongation onto trans, trans-farnesyl diphosphate (FPP) to produce undecaprenyl diphosphate (UPP), which is indispensable forExpand
  • 136
  • 8
  • PDF
Structure and function of cis-prenyl chain elongating enzymes.
All carbon skeletons of isoprenoids, whose chain lengths vary widely from geranyl diphosphate (C10) to natural rubber (C>10,000), are synthesized by sequential condensation of isopentenyl diphosphateExpand
  • 92
  • 8
Thermostable farnesyl diphosphate synthase of Bacillus stearothermophilus: molecular cloning, sequence determination, overproduction, and purification.
The structural gene for thermostable farnesyl diphosphate synthase from Bacillus stearothermophilus was cloned, sequenced, and overexpressed in Escherichia coli cells. A 1,260-nucleotide sequence ofExpand
  • 109
  • 7
Molecular analysis of cis-prenyl chain elongating enzymes.
Recent isolation of the gene for an undecaprenyl diphosphate synthase has disclosed the structures of many kinds of cis-prenyl chain elongating enzymes. Not only the primary structure but also theExpand
  • 71
  • 7
Manipulation of prenyl chain length determination mechanism of cis‐prenyltransferases
The carbon backbones of Z,E‐mixed isoprenoids are synthesized by sequential cis‐condensation of isopentenyl diphosphate (IPP) and an allylic diphosphate through actions of a series of enzymes calledExpand
  • 54
  • 7
Conversion from Farnesyl Diphosphate Synthase to Geranylgeranyl Diphosphate Synthase by Random Chemical Mutagenesis (*)
Prenyltransferases catalyze the consecutive condensation of isopentenyl diphosphate (IPP) with allylic diphosphates to produce prenyl diphosphates whose chain lengths are absolutely determined byExpand
  • 125
  • 5
Purification and primary structure of a new guanylic acid specific ribonuclease from Pleurotus ostreatus.
A guanine nucleotide-specific RNase (RNase Po1) was isolated from caps of the fruit bodies of Pleurotus ostreatus. RNase Po1 is most active towards RNA at pH 8.0. The effect of heating on the molarExpand
  • 51
  • 5
KaPPA-View. A Web-Based Analysis Tool for Integration of Transcript and Metabolite Data on Plant Metabolic Pathway Maps1[w]
The application of DNA array technology and chromatographic separation techniques coupled with mass spectrometry to transcriptomic and metabolomic analyses in plants has resulted in the generation ofExpand
  • 141
  • 4