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Phosphorylation of Munc-18/n-Sec1/rbSec1 by Protein Kinase C
It is shown that recombinant Munc-18 is phosphorylated by conventional PKC in a Ca- and phospholipid-dependent manner in a cell-free system and this results suggest that the PKC-catalyzed phosphorylation of Munm-18 plays an important role in regulating the interaction of MunC-18 with syntaxin and thereby the docking and/or the fusion of synaptic vesicles with the presynaptic plasma membrane.
A nomenclature for the mammalian flavin-containing monooxygenase gene family based on amino acid sequence identities.
The purpose of the proposed nomenclature is to provide for the unambiguous identification of orthologous forms of mammalian FMOs, regardless of the species or tissue in question.
A receptor-like protein-tyrosine phosphatase PTPzeta/RPTPbeta binds a heparin-binding growth factor midkine. Involvement of arginine 78 of midkine in the high affinity binding to PTPzeta.
Results suggested that Arg78 in midkine plays an essential role in high affinity binding to PTPzeta by interacting with the chondroitin sulfate portion of this receptor.
Highly sensitive peptide-4-methylcoumaryl-7-amide substrates for blood-clotting proteases and trypsin.
Seventy-four peptide amides of 7-amino-4-methylcoumarin of the type Boc-Xaa-Yaa-Arg-NH-Mec were newly synthesized and tested to find specific substrates for blood-clotting proteases and trypsin, with Ala and Pro found to be most sensitive substrates.
Studies on adrenal steroid hydroxylases. Molecular and catalytic properties of adrenodoxin reductase (a flavoprotein).
  • J. Chu, T. Kimura
  • Biology, Chemistry
    The Journal of biological chemistry
  • 25 March 1973
Abstract Adrenodoxin reductase, purified to near homogeneity from bovine adrenal glands, is an FAD-containing protein which contains 1 mole of FAD per mole of enzyme, and 2% (w/w) hexose. The
The Roles of Carbohydrate Chains of the β-Subunit on the Functional Expression of Gastric H+,K+-ATPase*
The effects of a series of progressive removals of carbohydrate chains on the H+K+-ATPase activity were cumulative, and removal of all carbohydrate chains resulted in the complete loss of H+,K-ATpase activity.
Calciseptine binding to a 1,4-dihydropyridine recognition site of the L-type calcium channel of rat synaptosomal membranes.
Observations indicate that CaS shares the properties of 1,4-dihydropyridine derivatives, and allosterically modulates the binding of other L-type Ca2+ channel ligands.
Carboxyl-terminal parathyroid hormone fragments stimulate osteoclast-like cell formation and osteoclastic activity.
It is indicated that C-terminal PTH fragments stimulate osteoclast-like cell formation as well as bone-resorbing activity by mature osteoclasts in the presence of osteoblasts and accelerate osteoclastic cell formation from hemopoietic blast cells in the absence of osteoblastasts.
New fluorogenic substrates for alpha-thrombin, factor Xa, kallikreins, and urokinase.
Twenty peptide-4-methylcoumarin amides (MCA) were newly synthesized and tested as possible substrates for alpha-thrombin, factor Xa, kallikreins, urokinase, and plasmin. These fluorogenic peptides