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Cloning, sequencing, and expression of the gene encoding Clostridium paraputrificum chitinase ChiB and analysis of the functions of novel cadherin-like domains and a chitin-binding domain
TLDR
Deletion analysis showed clearly that the CBD of ChiB plays an important role in hydrolysis of native chitin but not processed chitIn such as colloidal chit in C. paraputrificum.
Cloning of genes involved in carbazole degradation of Pseudomonas sp. strain CA10: nucleotide sequences of genes and characterization of meta-cleavage enzymes and hydrolase
TLDR
Alignment analysis and the phylogenetic tree indicate that CarC has greatest homologies with hydrolases involved in the monoaromatic compound degradation pathway, suggesting the possibility that Car c is a novel type of hydrolase.
Electrostatic mechanism of chromatin folding.
Purification, Characterization, and Molecular Cloning of Acidophilic Xylanase from Penicillium sp.40
TLDR
The gene encoding XynA (xynA) was isolated from the genomic library of Penicillium sp.
Cloning, DNA sequencing, and expression of the gene encoding Clostridium thermocellum cellulase CelJ, the largest catalytic component of the cellulosome
TLDR
The results strongly suggest that CelJ is equivalent to S2, which was identified as the largest catalytic component in the cellulosome of C. thermocellum YS.
Characterization of the Cellulolytic Complex (Cellulosome) from Ruminococcus albus
TLDR
Results strongly suggest that the cellulolytic complex produced by R. albus resembles the cellulosome specified for the celluloytic complex of several clostridia, which is formed by the interaction between a dockerin domain of catalytic components and a cohesindomain of a scaffolding protein.
Sequence of xynC and properties of XynC, a major component of the Clostridium thermocellum cellulosome
TLDR
Immunological and N-terminal amino acid sequence analyses disclosed that the full-length XynC is one of the major components of the C. thermocellum cellulosome.
Importance of the Carbohydrate-Binding Module of Clostridium stercorarium Xyn10B to Xylan Hydrolysis
TLDR
This is the first report of a family 9 CBM with an affinity for insoluble xylan in addition to crystalline cellulose and the ability to increase hydrolytic activity toward insolublexylan.
Molecular breeding of transgenic rice expressing a xylanase domain of the xynA gene from Clostridium thermocellum
TLDR
The results indicated that high levels of xylanase can be produced in rice plants.
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