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Nanostructured Hydrogels for Three‐Dimensional Cell Culture Through Self‐Assembly of Fluorenylmethoxycarbonyl–Dipeptides
TLDR
A number of short peptide amphiphiles consisting of dipeptides linked to fluorenylmethoxycarbonyl spontaneously form fibrous hydrogels under physiological conditions, and the gels support the three-dimensional cell culture of chondrocytes.
Biocatalysis with Thermostable Enzymes: Structure and Properties of a Thermophilic ‘ene’‐Reductase related to Old Yellow Enzyme
TLDR
The crystal structure of a thermophilic “ene” reductase (TOYE) isolated from Thermoanaerobacter pseudethanolicus E39 reveals a tetrameric enzyme and an active site that is relatively large compared to most other structurally determined and related Old Yellow Enzymes.
Missense mutations that cause Van der Woude syndrome and popliteal pterygium syndrome affect the DNA-binding and transcriptional activation functions of IRF6
TLDR
The sequence to which IRF6 binds is determined and this sequence is used to analyse the effect of VWS- and PPS-associated mutations in the DNA-binding domain of IRf6 and it is demonstrated that IRF 6 functions as a co-operative transcriptional activator and that mutations inThe protein interaction domain ofIRF6 disrupt this activity.
Structural basis for complement factor H–linked age-related macular degeneration
TLDR
The crystal structure of the region of FH containing the polymorphic amino acid His402 in complex with an analogue of the glycosaminoglycans (GAGs) that localize the complement regulator on the cell surface demonstrates direct coordination of ligand by the disease-associated polymorphic residue, providing a molecular explanation of the genetic observation.
The Angiogenic Inhibitor Long Pentraxin PTX3 Forms an Asymmetric Octamer with Two Binding Sites for FGF2*
TLDR
Biophysical analyses reveal that PTX3 is composed of eight identical protomers, associated through disulfide bonds, forming an elongated and asymmetric, molecule with two differently sized domains interconnected by a stalk, explaining both its quaternary organization and how this is required for its antiangiogenic function.
TSG-6 Inhibits Neutrophil Migration via Direct Interaction with the Chemokine CXCL8
TLDR
TSG-6 is identified as a CXCL8-binding protein, making it, to the authors' knowledge, the first soluble mammalian chemokine-bindingprotein to be described to date and revealed a potential mechanism whereby TSG- 6 mediates its anti-inflammatory and protective effects.
Heparan Sulfate Regulates Fibrillin-1 N- and C-terminal Interactions*
TLDR
Fibrillin-1 N- and C-terminal interactions with heparin/heparan sulfate directly influence cell behavior, whereas C- Terminals with heParin/ hepara- sulfate regulate elastin deposition.
Molecular basis of coiled-coil oligomerization-state specificity
TLDR
The important and unresolved question why the presence of particular oligomerization-state determinants within a coiled coil does frequently not correlate with its topology is addressed and an unexpected, general link between coiled-coil oligomerized-state specificity and trigger sequences is found.
Metal Ion-dependent Heavy Chain Transfer Activity of TSG-6 Mediates Assembly of the Cumulus-Oocyte Matrix*
TLDR
The tertiary structure of the CUB module from human TSG-6 is determined, identifying a calcium ion-binding site and chelating glutamic acid residue that mediate the formation of HC·TSG- 6.
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