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FRET imaging
TLDR
A revised photophysical framework for the phenomenon is introduced and a systematic catalog of FRET techniques adapted to imaging systems is provided, including new approaches proposed as suitable prospects for implementation.
A mutant EGF‐receptor defective in ubiquitylation and endocytosis unveils a role for Grb2 in negative signaling
TLDR
It is reported that defective Tyr1045 of EGFR, an inducible c‐Cbl docking site, enhances the mitogenic response to EGF, and Grb2 can terminate signal transduction by accelerating c-Cbl‐dependent sorting of active tyrosine kinases to destruction.
Release of long-range tertiary interactions potentiates aggregation of natively unstructured alpha-synuclein.
TLDR
Stabilization of the native, autoinhibitory structure of alphaS constitutes a potential strategy for reducing or inhibiting oligomerization and aggregation in Parkinson's disease.
Decreased accessibility and lack of activation of ErbB2 in JIMT-1, a herceptin-resistant, MUC4-expressing breast cancer cell line.
TLDR
It is concluded that masking of erbB2 in JIMT-1 leads to diminished Herceptin binding and isolation of erBB2 from its normal interaction and activation partners, which may explain its HerceptIn-resistant phenotype characterizing both the low and high Hercept in binding subpopulations.
Oligomerization of epidermal growth factor receptors on A431 cells studied by time-resolved fluorescence imaging microscopy. A stereochemical model for tyrosine kinase receptor activation
TLDR
It is proposed that the transmission of the external ligand-binding signal to the cytoplasmic domain is effected by a concerted relative rotational rearrangement of the monomeric units comprising the dimeric receptor, thereby potentiating a mutual activation of the tyrosine kinase domains.
Quantum dot ligands provide new insights into erbB/HER receptor–mediated signal transduction
TLDR
It is demonstrated that EGF-QDs are highly specific and potent in the binding and activation of the EGF receptor (erbB1), being rapidly internalized into endosomes that exhibit active trafficking and extensive fusion.
Ligand-Induced Internalization of the p75 Neurotrophin Receptor: A Slow Route to the Signaling Endosome
TLDR
NGF induced endosomal association of p75 and its MAGE interactors, necdin and NRAGE suggest that signaling endosome containing activated p75 are involved in neurotrophin signaling, and that such endosomes may be temporally and spatially distinct from those containing trk receptors.
Impact of the acidic C-terminal region comprising amino acids 109-140 on alpha-synuclein aggregation in vitro.
TLDR
The effects of the C-terminus on aggregation cannot be rationalized merely by a contribution to the protein net charge, but rather suggest a specific role of aa109-140 in the regulation of aggregation, presumably involving formation of intramolecular contacts.
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