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Myosin VI is a processive motor with a large step size
Myosin VI is a molecular motor involved in intracellular vesicle and organelle transport. To carry out its cellular functions myosin VI moves toward the pointed end of actin, backward in relation toExpand
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The chromatin remodeler ACF acts as a dimeric motor to space nucleosomes
Evenly spaced nucleosomes directly correlate with condensed chromatin and gene silencing. The ATP-dependent chromatin assembly factor (ACF) forms such structures in vitro and is required forExpand
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Role of the lever arm in the processive stepping of myosin V
Myosin V is a two-headed molecular motor that binds six light chains per heavy chain, which creates unusually long lever arms. This motor moves processively along its actin track in discrete 36-nmExpand
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A force-dependent state controls the coordination of processive myosin V.
Myosin V is an efficient processive molecular motor. Recent experiments have shown how the structure and kinetics of myosin V are specialized to produce a highly processive motor capable of takingExpand
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Production of a Recombinant Bovine Enterokinase Catalytic Subunit in the Methylotrophic Yeast Pichia pastoris
We describe the heterologous expression of a 26.3 kD protein containing the catalytic domain of bovine enterokinase (EKL) in the methylotrophic yeast Pichia pastoris. A highly active protein isExpand
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Building and using optical traps to study properties of molecular motors.
Publisher Summary This chapter describes the building and using of optical traps to study the properties of molecular motors. Optical trapping experiments very directly measure the velocity, force,Expand
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Genome-scale cloning and expression of individual open reading frames using topoisomerase I-mediated ligation.
The in vitro cloning of DNA molecules traditionally uses PCR amplification or site-specific restriction endonucleases to generate linear DNA inserts with defined termini and requires DNA ligase toExpand
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Nucleotide pocket thermodynamics measured by EPR reveal how energy partitioning relates myosin speed to efficiency.
We have used spin-labeled ADP to investigate the dynamics of the nucleotide-binding pocket in a series of myosins, which have a range of velocities. Electron paramagnetic resonance spectroscopyExpand
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Combining EPR with fluorescence spectroscopy to monitor conformational changes at the myosin nucleotide pocket.
We used spin-labeled nucleotide analogs and fluorescence spectroscopy to monitor conformational changes at the nucleotide-binding site of wild-type Dictyostelium discoideum (WT) myosin and aExpand
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