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Detection in situ and characterization of lignin in the G-layer of tension wood fibres of Populus deltoides
Immunochemical labelling provides the first visualization in planta of lignin structures within the G-layer of tension wood fibres, and patterns of distribution of syringyl epitopes indicate thatSyringyl lignIn is deposited more intensely in the later phase of fibre secondary wall assembly, and is under specific spatial and temporal regulation targeted differentially throughout cell wall layers. Expand
Discriminating the indistinguishable sapwood from heartwood in discolored ancient wood by direct molecular mapping of specific extractives using time-of-flight secondary ion mass spectrometry.
Time-of-flight secondary ion mass spectrometry (TOF-SIMS) analysis of specific chemical substances retained for approximately 1300 years after felling demonstrated the presence of sapwood in a discolored ancient architectural wood of Hinoki cypress. Expand
Evidence for involvement of the phenylpropanoid pathway in the biosynthesis of the norlignan agatharesinol.
In order to study the biosynthesis of agatharesinol, a norlignan, l-phenylalanine and trans-cinnamic acid and 13C-labeled precursors were administered to fresh sapwood sticks of Cryptomeria japonica, and assimilation of the labeled precursor into agathresinol was clearly detected. Expand
Ready chemical conversion of acid hydrolysis lignin into water-soluble lignosulfonate II: Hydroxymethylation and subsequent sulfonation of phenolized lignin model compounds
Highly condensed lignin can be transformed by three reactions — phenolation, hydroxymethylation, and neutral sulfonation — to water-soluble lignosulfonate. To elucidate reactivities and products inExpand
Direct mapping of morphological distribution of syringyl and guaiacyl lignin in the xylem of maple by time-of-flight secondary ion mass spectrometry.
Time-of-flight secondary ion mass spectrometry (TOF-SIMS) is used to directly map guaiacyl (G) and syringyl (S) lignin units in several successive growth rings of the maple xylem to indicate that TOF- SIMS allows direct visualization of lignIn composition in plant tissues. Expand
Localization of ferruginol, a diterpene phenol, in Cryptomeria japonica heartwood by time-of-flight secondary ion mass spectrometry
Gas chromatography-mass spectrometry of the n-hexane extract demonstrated that ferruginol, a diterpene phenol, constituted one of the predominant constituents of the extract and was detected uniformly in the tracheid cell walls, in the cell walls of the axial parenchyma cells and ray parenchma cells, and also inside these parenchema cells. Expand
The cryo‐TOF‐SIMS/SEM system for the analysis of the chemical distribution in freeze‐fixed Cryptomeria japonica wood
Initially, we investigated the distribution of chemicals in dried wood samples using a standard TOF-SIMS and obtained two-dimensional images of chemical distribution in various wood samples. However,Expand
Chemical differences between sapwood and heartwood of Chamaecyparis obtusa detected by ToF-SIMS
Abstract Time-of-flight secondary ion mass spectrometry (ToF-SIMS) was used to investigate the distribution of elements, Na, Mg, Al, K, and Ca, and lignin, in the contiguous growth rings includingExpand
Preparation of antibody against agatharesinol, a norlignan, using a hapten-carrier conjugate.
The antiserum produced reacts most strongly with agatharesinol and recognizes norlignans almost selectively among Sugi heartwood extractives, indicating the successful production of anti-agathares inol antibodies. Expand
Characterization of Physiological Functions of Sapwood Fluctuation of Extractives in the Withering Process of Japanese Cedar Sapwood
In order to characterize the physiological functions of sapwood of needle-leaved tree, ten definite sugar and phenolic constituent contents were periodically determined among outer, middle and innerExpand