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Insights into the complex regulation of rpoS in Borrelia burgdorferi
A model for regulation of rpoS has been proposed which provides mechanisms for multiple signalling pathways to modulate the expression of the σS regulon in B. burgdorferi. Expand
Novel Features of the Polysaccharide-Digesting Gliding Bacterium Flavobacterium johnsoniae as Revealed by Genome Sequence Analysis
Analysis of the genome identified genes related to sprB that may encode alternative adhesins used for movement over different surfaces and genes predicted to encode components of a multienzyme nonribosomal peptide synthetase, as were novel aspects of gene regulation. Expand
Transcriptional regulation at a distance in bacteria.
  • H. Xu, T. Hoover
  • Biology, Medicine
  • Current opinion in microbiology
  • 1 April 2001
Significant advances have been made over the last few years towards understanding the mechanisms by which bacterial enhancer-binding proteins activate transcription, but important aspects of these mechanisms are still poorly defined. Expand
Synergistic transcriptional activation by one regulatory protein in response to two metabolites
In vitro transcription assays demonstrated that two metabolites of aromatic compound catabolism, benzoate and cis,cis-muconate, act synergistically to activate gene expression and may represent an important, and possibly widespread, feature of transcriptional control. Expand
Deciphering bacterial flagellar gene regulatory networks in the genomic era.
This work reviews the literature on the expression and activity of several known master regulators, including FlhDC, CtrA, VisNR, FleQ, FlrA, FlaK, LafK, SwrA and MogR, and examines the Department of Energy Joint Genomes Institute database to make predictions about the distribution of these regulators. Expand
Survival in Nuclear Waste, Extreme Resistance, and Potential Applications Gleaned from the Genome Sequence of Kineococcus radiotolerans SRS30216
The results highlight the remarkable ability of K radiotolerans to withstand environmental extremes and suggest that in situ bioremediation of organic complexants from high level radioactive waste may be feasible. Expand
The integration host factor stimulates interaction of RNA polymerase with NIFA, the transcriptional activator for nitrogen fixation operons
IHF greatly stimulates NIFA-mediated activation of nifH transcription in vitro and thus that the two factors are functionally synergistic, and the IHF-induced bend may facilitate productive contacts between NIFA and sigma 54 holoenzyme that lead to the formation of open complexes. Expand
The structural basis for regulated assembly and function of the transcriptional activator NtrC.
X-ray solution scattering and electron microscopy structures of the activated, full-length nitrogen-regulatory protein C (NtrC) are reported showing a novel mechanism for regulation of AAA+ ATPase assembly via the juxtaposition of the receiver domains and ATPase ring. Expand
Role of integration host factor in stimulating transcription from the sigma 54-dependent nifH promoter.
With purified NTRC and the substitution construct, it is demonstrated that stimulation by IHF in a purified transcription system was comparable to that in a crude coupled transcription-translation system, indicating that the stimulation in the crude system could be accounted for by I HF. Expand
ATP ground- and transition states of bacterial enhancer binding AAA+ ATPases support complex formation with their target protein, sigma54.
SAS-based solution structures of the ATPase domain of the EBP NtrC1 from Aquifex aeolicus in different nucleotide states indicate that ATP binding promotes a conformational change that stabilizes complexes between EBPs and sigma54, while subsequent hydrolysis and phosphate release drive the conformationalchange needed to open the polymerase/promoter complex. Expand