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Non-invasive quantitative detection and applications of non-toxic, S65T-type green fluorescent protein in living plants.
Green fluorescent protein (GFP) has emerged as a powerful new tool in a variety of organisms. An engineered sGFP(S65T) sequence containing optimized codons of highly expressed eukaryotic proteins hasExpand
Excellent Salinity Tolerance of Mozambique Tilapia (Oreochromis mossambicus): Elevated Chloride Cell Activity in the Branchial and Opercular Epithelia of the Fish Adapted to Concentrated Seawater
Abstract Changes in morphology and cellular activity of the chloride cells in branchial and opercular epithelia were examined in tilapia, Oreochromis mossambicus, adapted to fresh water (FW),Expand
Amidated fish ghrelin: purification, cDNA cloning in the Japanese eel and its biological activity.
We purified ghrelin from stomach extracts of a teleost fish, the Japanese eel (Anguilla japonica) and found that it contained an amide structure at the C-terminal end. Two molecular forms of ghrelinExpand
Changes in cortisol and thyroid hormone concentrations during early development and metamorphosis in the Japanese flounder, Paralichthys olivaceus.
Both cortisol and thyroid hormones were detected in newly fertilized eggs of the Japanese flounder, Paralichthys olivaceus. Mean cortisol levels ranged around 2.5 ng/g wet weight. CortisolExpand
Identification of tilapia ghrelin and its effects on growth hormone and prolactin release in the tilapia, Oreochromis mossambicus.
We have identified ghrelin and cDNA encoding precursor protein from the stomach of a euryhaline tilapia, Oreochromis mossambicus. The sequence of 20-amino acid tilapia ghrelin isExpand
Switching of Na+, K+-ATPase isoforms by salinity and prolactin in the gill of a cichlid fish.
We identified and investigated the changes in expression of two gill Na(+), K(+)-ATPase α-subunit isoforms (α-1a and α-1b) in relationship with salinity acclimation in a cichlid fish, MozambiqueExpand
Osmoregulatory actions of growth hormone and its mode of action in salmonids: A review
Osmoregulatory actions of growth hormone (GH) and its mode of action in salmonids are reviewed. We present evidence suggesting that insulin-like growth factor I (IGF-I) mediates some of the actionsExpand
Prolactin receptor, growth hormone receptor, and putative somatolactin receptor in Mozambique tilapia: tissue specific expression and differential regulation by salinity and fasting.
In fish, pituitary growth hormone family peptide hormones (growth hormone, GH; prolactin, PRL; somatolactin, SL) regulate essential physiological functions including osmoregulation, growth, andExpand
Calcium uptake in the skin of a freshwater teleost.
The skin, particularly the opercular membrane of some teleosts, contains mitochondrion-rich "chloride" cells and has been widely used as a model to study branchial salt-extrusion mechanisms inExpand
The spectrum of prolactin action in teleosts.
  • T. Hirano
  • Biology, Medicine
  • Progress in clinical and biological research
  • 1986