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Studies on the biosynthesis of fosfomycin. 4. The biosynthetic origin of the methyl group of fosfomycin.
By bioconversion experiments using two FM non-producing mutants of Streptomyces wedmorensis, NP-7 and A16, it is shown that the epoxide ring of FM was formed by dehydrogenation of 2-hydroxypropylphosphonic acid (HPP)10'11*. Expand
Cloning and nucleotide sequence of fosfomycin biosynthetic genes of Streptomyces wedmorensis
The biosynthetic pathway for production of the antibiotic fosfomycin by Streptomyces wedmorensis consists of four steps including the formation of a C-P bond and an epoxide and the presence of ten open reading frames, including the four biosynthesis genes and six genes with unknown functions. Expand
Nucleotide sequence of fortimicin KL1 methyltransferase gene isolated from Micromonospora olivasterospora, and comparison of its deduced amino acid sequence with those of methyltransferases involved
PnAA was directly methylated by a nucleophilic attack of the methyl anion derived from methylcobalamin catalyzed by PnAA methyltransferase, and the nucleotide sequences of the genes encoding P-methyltransferase were determined. Expand
Purification and characterization of phosphoenolpyruvate phosphomutase from Pseudomonas gladioli B-1
The C-P bond-forming activity of PEPPM was confirmed with a purified protein, and the equilibrium of this reaction favors the formation of the phosphate ester by cleaving the C-p bond of PnPy, but theC-P Bond forming reaction is physiologically significant. Expand
Carboxyphosphonoenolpyruvate phosphonomutase, a novel enzyme catalyzing C-P bond formation
An enzyme catalyzing the formation of an unusual C-P bond that is involved in the biosynthesis of the antibiotic bialaphos (BA) was isolated from the cell extract of a mutant (NP71) of StreptomycesExpand
Promothiocins A and B novel thiopeptides with a tipA promoter inducing activity produced by Streptomyces sp. SF2741.
The tipA promoter was inserted in a promoter probe vector so that it controlled the expression of a kanamycin resistance gene (pAK114) so that S. lividans became highly resistant to the antibiotic in the presence of very small amounts of thiostrepton. Expand
Geninthiocin was isolated from the mycelium of Streptomyces sp. DD84 as a tip A promoter inducing substance. Based on various NMR studies, its structure was established as a thiopeptide with oxazoleExpand
Fosfomycin monophosphate and fosfomycin diphosphate, two inactivated fosfomycin derivatives formed by gene products of fomA and fomB from a fosfomycin producing organism Streptomyces wedmorensis.
The FMgene cluster was investigated to identify a gene(s) which confers FMresistance to E. coli and it was revealed that bothfomA andfomB, formerly called as orfA and orfB9\ respectively, and characterized as FM-resistance genes, were required for complete FMbiosynthesis. Expand