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Preparation of unilamellar liposomes of intermediate size (0.1-0.2 mumol) by a combination of reverse phase evaporation and extrusion through polycarbonate membranes.
The procedure is relatively simple, rapid, and yields almost quantitative recovery of vesicles that encapsulate a large percentage of the total aqueous space. Expand
Lipid-DNA complexes induce potent activation of innate immune responses and antitumor activity when administered intravenously.
DNA complexed to cationic liposomes becomes highly immunostimulatory and capable of inducing strong antitumor activity when administered systemically, and was dependent on both NK cells and IFN-gamma. Expand
Factors influencing the efficiency of cationic liposome-mediated intravenous gene delivery
The level of gene expression per microgram of DMA taken up per tissue was 1,000-fold higher in lung than in liver, indicating that in addition to issues of delivery and retention of injected DNA, tissue-specific host factors also play a central role in determining the efficiency of expression. Expand
Non-replicating Epstein-Barr Virus-based Plasmids Extend Gene Expression and Can Improve Gene Therapy in Vivo *
A gene therapy regimen using non-replicating Epstein-Barr virus (EBV)-based expression plasmids that markedly prolongs gene expression at therapeutic levels and efficiently and repeatedly re-transfects immunocompetent hosts. Expand
New aspects of liposomes.
A novel series of amphiphilic imidazolinium compounds for in vitro and in vivo gene delivery.
Three catioinic amphiphiles based on the structure 1-[2-(acyloxy)ethyl]-2-alkyl(alkenyl)-3-(2-hydroxyethyl)imidazolinium chloride differ greatly in their ability to mediate in vivo and in vitro gene delivery. Expand
Proteoglycans Mediate Cationic Liposome-DNA Complex-based Gene Delivery in Vitro and in Vivo *
Modulation of proteoglycan expression may prove useful in controlling the efficiency of, as well as targeting the sites of, CLDC-based gene transfer in animals. Expand
Serum concentrations and analgesic effects of liposome-encapsulated and standard butorphanol tartrate in parrots.
The use of LEBT may allow for substantial improvement in long-term pain relief without subjecting birds to the stress of handling and multiple daily injections. Expand
Equivalent pore estimate for the alveolar-airway barrier in isolated dog lung.
In high-pressure pulmonary edema, lung interstitial and air space edema liquids have equal protein concentrations (Am. J. Physiol. 231: 1466, 1976). This suggests that the alveolar-airway barrierExpand