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Activation of Helicobacter pylori VacA Toxin by Alkaline or Acid Conditions Increases Its Binding to a 250-kDa Receptor Protein-tyrosine Phosphatase β*
Helicobacter pylori, a Gram-negative gastric bacterium, secretes VacA, a cytotoxin that causes vacuolar degeneration of susceptible cells. Velocity sedimentation analysis showed that treatment ofExpand
Salmonella enteritidis FliC (Flagella Filament Protein) Induces Human β-Defensin-2 mRNA Production by Caco-2 Cells*
Antimicrobial peptides are crucial for host defense at mucosal surfaces. Bacterial factors responsible for induction of human β-defensin-2 (hBD-2) mRNA expression in Caco-2 human carcinoma cells wereExpand
Vacuolating cytotoxin purified from Helicobacter pylori causes mitochondrial damage in human gastric cells.
We investigated the effects of vacuolating cytotoxin (VacA) prepared from Helicobacter pylori on the metabolism of gastric epithelial cells, AZ-521. VacA caused the ATP levels to decrease in aExpand
Interaction of pleurocidin and its analogs with phospholipid membrane and their antibacterial activity.
A 25-mer cationic peptide pleurocidin, isolated from the winter flounder, has broad antibacterial activity. To clarify the structure-activity relationship, its properties and biological activity wereExpand
Antibacterial activity of bactenecin 5 fragments and their interaction with phospholipid membranes
Bactenecin 5 (Bac 5) is an antibacterial 43mer peptide isolated from bovine neutrophils. It consists of an Arg‐rich N‐terminal region and successive repeats of Arg‐Pro‐Pro‐Ile (or Phe). WeExpand
Helicobacter pylori vacuolating cytotoxin binds to the 140-kDa protein in human gastric cancer cell lines, AZ-521 and AGS.
To investigatie a potential mechanism of how Helicobacter pylori establishes infection, we purified a lot of vacuolating toxin (VacA) from supernatant of H. pylori ATCC49503 (tox+ strain 60190). WeExpand
Interaction of alpha-helical peptides with phospholipid membrane: effects of chain length and hydrophobicity of peptides.
To investigate the interaction of amphiphilic alpha-helical peptides with phospholipid membranes, we synthesized Ac-(Leu-Ala-Arg-Leu)3-NHCH3 (4[3]) and three derivatives, in which the chain lengthExpand
Small‐angle X‐ray scattering study on CEL‐III, a hemolytic lectin from Holothuroidea Cucumaria echinata, and its oligomer induced by the binding of specific carbohydrate
Hemolytic lectin CEL‐III from a marine invertebrate Cucumaria echinata forms an oligomer upon binding of specific carbohydrate such as lactose at high pH values and in the presence of highExpand
Fishing for bioactive substances from scorpionfish and some sea urchins.
Venom proteins from the dorsal spine of two scorpionfish, Hypodytes rubripinnis and Synanceia verrucosa were assayed for mitogenicity and cytotoxicity. The two venoms had both mitogenic and cytotoxicExpand
The enhancing effect of anionic alpha-helical peptide on cationic peptide-mediating transfection systems.
A peptide consisting of 12 amino acids including 3 glutamic acids (LAEL-LAEL-LAEL; 4(3)E) underwent pH-dependent conformational change from random coil to alpha-helix when the pH was decreased fromExpand