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An Efficient Multistrategy DNA Decontamination Procedure of PCR Reagents for Hypersensitive PCR Applications
TLDR
A versatile multistrategy decontamination procedure for PCR reagents is developed that allows efficient reagent decontamination while preserving the efficiency of PCR amplification of minute quantities of DNA.
An Antisense RNA Regulates the Bidirectional Silencing Property of the Kcnq1 Imprinting Control Region
TLDR
The antisense RNA plays a key role in the silencing function of the ICR, and is characterized by genomic footprinting and tested the functional consequence of various deletions of these binding sites in an episome-based system.
Glucocorticoid‐induced DNA demethylation and gene memory during development
TLDR
Genomic footprinting analysis shows that the glucocorticoid receptor uses local DNA demethylation as one of several steps to recruit transcription factors in hepatoma cells and appears to contribute to the fine‐tuning of the enhancer and to the memorization of a regulatory event during development.
Messenger RNA deadenylylation precedes decapping in mammalian cells.
TLDR
It is indicated that decapping is preceded by a shortening of the poly(A) tail in mammalian cells, as it is in yeast, suggesting that this mRNA degradation pathway is conserved throughout eukaryotic evolution.
DNA physical properties determine nucleosome occupancy from yeast to fly
TLDR
The model uses only basic physical properties to describe the wrapping of DNA around the histone core, yet it captures a substantial part of chromatin's structural complexity, leading to a much better prediction of nucleosome occupancy than methods based merely on periodic curved DNA motifs.
Past climate changes, population dynamics and the origin of Bison in Europe
TLDR
The paleogenetic analysis of bison remains from the last 50,000 years reveals the influence of climate changes on the dynamics of the various bison populations in Europe, only one of which survived into the Holocene, where it experienced severe reductions in its genetic diversity.
Cell-type specific activity of two glucocorticoid responsive units of rat tyrosine aminotransferase gene is associated with multiple binding sites for C/EBP and a novel liver-specific nuclear factor
TLDR
DNAase I footprinting and gel mobility shift analyses reveal a complex array of contiguous and overlapping sites for cell type-specific DNA binding proteins in the remote glucocorticoid responsive units.
The expression cassette determines the functional activity of ribozymes in mammalian cells by controlling their intracellular localization.
TLDR
It is demonstrated that the ribozyme expression cassette determines its intracellular localization and, hence, its corresponding functional activity.
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