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Cloning and characterization of the cDNAs for human and rabbit interleukin-1 precursor.
DNA sequence complementary to the mRNA for rabbit interleukin-1 precursor (preIL-1) has been cloned from the cDNA library constructed using partially purified poly(A)+RNA from induced rabbit alveolar
Cloning and sequencing of the cDNA for human monocyte chemotactic and activating factor (MCAF).
The amino acid sequence of MCAF showed 25-55% homology with other members of an inducible cytokine family, including macrophage inflammatory protein and some putative polypeptide mediators known as JE, LD78, RANTES and TCA-3, suggesting that MCAf is a member of family of factors involved in immune and inflammatory responses.
Efficient production of a human tumour necrosis factor in Escherichia coli.
To examine the effect of altering the nucleotide sequence of the Shine-Dalgarno (SD) region and the spacer sequence between the SD sequence and the AUG translation start signal, several plasmids were
Production and characterization of recombinant human neutrophil chemotactic factor.
Recombinant human NCF was found to be biologically active and to exhibit chemotactic activity for human neutrophils in vitro and cause a neutrophil infiltration in vivo in mice.
Amino acid sequence of mammalian elongation factor 2 deduced from the cDNA sequence: homology with GTP-binding proteins.
The sequence provides direct evidence that diphthamide (2-carboxy-amido-3-(trimethylammonio)propyl]histidine), the site of ADP-ribosylation by diphtheria toxin, is produced by post-translational modification of a histidine residue in the primary translational product.
Complete nucleotide sequence of the gene for human interleukin 1 alpha.
The nucleotide sequence reported here showed only one nucleotide substitution in the 3'-untranslated exon in comparison with the nucleotide sequences of the cDNA from HL-60, a promyelocytic leukemia cell line, previously cloned and sequenced in the authors' laboratories.
Molecular cloning and the complete nucleotide sequence of cDNA to mRNA for S-100 protein of rat brain.
A dot-blot hybridization of poly(A) RNA from the developing rat brains using a labeled cDNA showed a rapid increase in S-100 mRNA at 10-20 postnatal days and the homology between the nucleotide sequence of S- 100 and those of rat intestinal Ca-binding protein (ICaBP) and calmodulin (CaM) was examined.
Studies of nucleosides and nucleotides.: LVIII. Deamination of adenosine analogs with calf intestine adenosine deaminase☆
Several synthetic adeonosine analogs: 8-fluoro-, 8-azido-, 8-iodo-, 8-methylthioadenosine; 8-bromo-2′-deoxyadenosine, 8-bromoxylofuranosyladenine, 5′-benzoly-8-bromoadenosine; 8,2′-S-, 8,2′-O-,
Molecular cloning and nucleotide sequence of cDNA coding for rat brain cholecystokinin precursor.
A mixture of 14-mer oligodeoxynucleotides was used for the screening of a cDNA clone coding for a cholecystokinin (CCK) precursor from a c DNA library for rat brain microsomal poly(A)RNA, verified to contain a nearly full-length cDNA sequence coding for rat CCK precursor.
An autopsy case of acute porphyria with a decrease of both uroporphyrinogen I synthetase and ferrochelatase activities
An autopsy case of a 37-year-old woman with acute porphyria revealed a marked loss and degeneration of axons and myelin sheaths in the peripheral nervous system (PNS), and prominent central chromatolysis of the spinal anterior horn cells.