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Paradigm shift of the plasma membrane concept from the two-dimensional continuum fluid to the partitioned fluid: high-speed single-molecule tracking of membrane molecules.
TLDR
The high-speed single-molecule tracking methods are described, and a new model of a partitioned fluid plasma membrane and the involvement of the actin-based membrane-skeleton "fences" and anchored-transmembrane protein "pickets" in the formation of compartment boundaries are critically reviewed. Expand
Phospholipids undergo hop diffusion in compartmentalized cell membrane
TLDR
It is proposed that various transmembrane proteins anchored to the actin-based membrane skeleton meshwork act as rows of pickets that temporarily confine phospholipids. Expand
Accumulation of anchored proteins forms membrane diffusion barriers during neuronal polarization
TLDR
It is found that various membrane proteins anchored to the dense membrane skeleton function as rows of pickets, which even stop the overall diffusion of phospholipids, and may represent a universal mechanism for formation of diffusion barriers in the cell membrane. Expand
Oscillatory Control of Factors Determining Multipotency and Fate in Mouse Neural Progenitors
Oscillation Stabilizes the Progenitor State Transcription factors regulate fate choice between different neural lineages, but the same transcription factors are also expressed in neural progenitorExpand
Single-molecule imaging analysis of Ras activation in living cells.
TLDR
The single-molecule FRET method developed here provides a powerful technique to study the signal-transduction mechanisms of various G proteins, suggesting that Ras signal transduction requires more than simple collisions with effector molecules. Expand
Ultrafine membrane compartments for molecular diffusion as revealed by single molecule techniques.
TLDR
The results strongly indicate the necessity for the paradigm shift of the concept on the plasma membrane: from the two-dimensional fluid continuum model to the compartmentalized membrane model in which its constituent molecules undergo hop diffusion over the compartments. Expand
Rapid hop diffusion of a G-protein-coupled receptor in the plasma membrane as revealed by single-molecule techniques.
TLDR
It is found that muOR undergoes rapid hop diffusion over membrane compartments, which are likely delimited by the actin-based membrane-skeleton "fence or corrals" and its associated transmembrane protein "pickets", suggesting that the fence and picket models may also be applicable to G-protein coupled receptors. Expand
Dynamic organizing principles of the plasma membrane that regulate signal transduction: commemorating the fortieth anniversary of Singer and Nicolson's fluid-mosaic model.
TLDR
It is proposed that the cooperative action of the hierarchical three-tiered mesoscale (2-300 nm) domains--actin-membrane-skeleton induced compartments, raft domains, and dynamic protein complex domains--is critical for membrane function and distinguishes the plasma membrane from a classical Singer-Nicolson-type model. Expand
Hierarchical mesoscale domain organization of the plasma membrane.
TLDR
The basic molecular interactions required for the signal transduction function of the plasma membrane can be fundamentally understood and conveniently summarized as the cooperative actions of these mesoscale domains, where thermal fluctuations/movements of molecules and weak cooperativity play crucial roles. Expand
Relationship of lipid rafts to transient confinement zones detected by single particle tracking.
TLDR
Overall, the data demonstrate that there are raft-related domains present in certain regions of the plasma membrane of C3H cells, which can persist for tens of seconds. Expand
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