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The Composite Genome of the Legume Symbiont Sinorhizobium meliloti
The scarcity of usable nitrogen frequently limits plant growth. A tight metabolic association with rhizobial bacteria allows legumes to obtain nitrogen compounds by bacterial reduction of dinitrogenExpand
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Second symbiotic megaplasmid in Rhizobium meliloti carrying exopolysaccharide and thiamine synthesis genes.
Using physical and genetic data, we have demonstrated that Rhizobium meliloti SU47 has a symbiotic megaplasmid, pRmeSU47b, in addition to the previously described nod-nif megaplasmid pRmeSU47a. ThisExpand
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The regulator gene phoB mediates phosphate stress‐controlled synthesis of the membrane lipid diacylglyceryl‐N,N,N‐trimethylhomoserine in Rhizobium (Sinorhizobium) meliloti
Bacteria react to phosphate starvation by activating genes involved in the transport and assimilation of phosphate as well as other phosphorous compounds. Some soil bacteria have evolved anExpand
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General transduction in Rhizobium meliloti.
General transduction by phage phi M12 in Rhizobium meliloti SU47 and its derivatives is described. Cotransduction and selection for Tn5 insertions which are closely linked to specific loci wereExpand
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The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti
Analysis of the 1,683,333-nt sequence of the pSymB megaplasmid from the symbiotic N2-fixing bacterium Sinorhizobium meliloti revealed that the replicon has a high gene density with a total of 1,570Expand
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Novel pathway for arsenic detoxification in the legume symbiont Sinorhizobium meliloti.
We report a novel pathway for arsenic detoxification in the legume symbiont Sinorhizobium meliloti. Although a majority of ars operons consist of three genes, arsR (transcriptional regulator), arsBExpand
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Analysis of C4‐dicarboxylate transport genes in Rhizobium meliloti
A 5.1 kbp DNA fragment was isolated which complemented C4‐dicarboxylate transport mutants (dct) of Rhizobium meliloti. Characterization of this fragment by subcloning, transposon mutagenesis, andExpand
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Genomes of the Symbiotic Nitrogen-Fixing Bacteria of Legumes1
Over the last several decades, there have been a large number of studies done on the genetics, biochemistry, physiology, ecology, and agronomics of the bacteria forming nitrogen-fixing symbioses withExpand
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The Divided Bacterial Genome: Structure, Function, and Evolution
SUMMARY Approximately 10% of bacterial genomes are split between two or more large DNA fragments, a genome architecture referred to as a multipartite genome. This multipartite organization is foundExpand
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A phosphate transport system is required for symbiotic nitrogen fixation by Rhizobium meliloti.
The bacterium Rhizobium meliloti forms N2-fixing root nodules on alfalfa plants. The ndvF locus, located on the 1,700-kb pEXO megaplasmid of R. meliloti, is required for nodule invasion and N2Expand
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