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[Quantitative proteomics of human blood exosomes].
Exosomes are extracellular membrane vesicles secreted by cells into biological fluids. The outer membrane of exosomes protects their content from degradation and contains markers of the parent cell.Expand
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200+ Protein Concentrations in Healthy Human Blood Plasma: Targeted Quantitative SRM SIS Screening of Chromosomes 18, 13, Y, and the Mitochondrial Chromosome Encoded Proteome.
This work continues the series of the quantitative measurements of the proteins encoded by different chromosomes in the blood plasma of a healthy person. Selected Reaction Monitoring with StableExpand
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Antigenicity and B-epitope mapping of hepatitis C virus envelope protein E2
Immunogenicity for laboratory animals (rabbits and mice) of the whole hepatitis C virus envelope proteins and their conserved as well as hypervariable HVR1 sites has been investigated. Rabbit immuneExpand
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Application of selected reaction monitoring and parallel reaction monitoring for investigation of HL-60 cell line differentiation
Targeted mass spectrometry represents a powerful tool for investigation of biological processes. The convenient approach of selected reaction monitoring using stable isotope-labeled peptide standardExpand
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Increased Sensitivity of Mass Spectrometry by Alkaline Two-Dimensional Liquid Chromatography: Deep Cover of the Human Proteome in Gene-Centric Mode.
Currently, great interest is paid to the identification of "missing" proteins that have not been detected in any biological material at the protein level (PE1). In this paper, using the UniversalExpand
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Quantitative target proteomics of chromosome 13 human blood plasma proteins
Quantitative proteomic analysis of 50 blood plasma samples of healthy volunteers who underwent a comprehensive medical examination and were found eligible for space flights was performed. As a resultExpand
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Use of DNA-Aptamers for Enrichment of Low Abundant Proteins in Cellular Extracts for Quantitative Detection by Selected Reaction Monitoring
The relationship between the amount of a target protein in a complex biological sample and its amount measured by selected reaction monitoring (SRM) mass spectrometry upon the affinity enrichment ofExpand
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Preparation of monospecific antibodies against isoform 2 of translation elongation factor 1A (eEF1A2)
Amino acid sequences of eukaryotic translation elongation factor isoform 1 (eEF1A1) and 2 (eEF1A2) were compared and two peptide fragments of eEF1A2 were chosen as linear antigenic determinants forExpand
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Electrochemical detection of protein post-translational modifications: Phosphorylation and nitration of amyloid-beta (1–16)
Abstract Post-translational modifications (PTM) such as O-phosphorylation of tyrosine (Tyr) and serine (Ser) and 3-nitration of Tyr amino acid residues of proteinaceous molecules were successfullyExpand
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[Use of DNA-aptamers for enrichment of low abundant proteins in cellular extracts for quntitative detection by selected reaction monitoring].
The relationship between the amount of a target protein in a complex biological sample and its amount measured by selected reaction monitoring (SRM) mass spectrometry upon the affinity enrichment ofExpand