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Recent advances in high-throughput quantitative bioanalysis by LC-MS/MS.
Pharmacokinetic Interaction between Ritonavir and Indinavir in Healthy Volunteers
With the altered pharmacokinetic profile, indinavir likely could be given as a b.i.d. combination regimen with ritonavir to improve patient compliance and thereby reduce treatment failures.
Historical review of sample preparation for chromatographic bioanalysis: pros and cons
Sample preparation is a major task in a regulated bioanalytical laboratory. The sample preparation procedure significantly impacts assay throughput, data quality, analysis cost, and employee…
Rapid, simultaneous determination of lopinavir and ritonavir in human plasma by stacking protein precipitations and salting-out assisted liquid/liquid extraction, and ultrafast LC-MS/MS.
Salting-out assisted liquid/liquid extraction with acetonitrile: a new high throughput sample preparation technique for good laboratory practice bioanalysis using liquid chromatography-mass…
- Jun Zhang, Huaiqin Wu, Elaine J Kim, T. El-Shourbagy
- ChemistryBiomedical chromatography : BMC
- 1 April 2009
A new sample preparation technique, salting-out assisted liquid-liquid extraction with acetonitrile, for high-throughput good laboratory practice sample analysis using LCMS, which indicates that the method is rapid, reliable and suitable for regulated bioanalysis.
High-throughput salting-out assisted liquid/liquid extraction with acetonitrile for the simultaneous determination of simvastatin and simvastatin acid in human plasma with liquid chromatography.
Simultaneous determination of a hydrophobic drug candidate and its metabolite in human plasma with salting-out assisted liquid/liquid extraction using a mass spectrometry friendly salt.
A strategy of plasma protein quantitation by selective reaction monitoring of an intact protein.
The assay has proven rugged and specific and has been employed to generate data in support of preclinical studies and could be used for the development of bioanalytical assays to provide preclinical and clinical support for other protein drug candidates and, furthermore, for the validation of biomarkers discovered from proteomic research.
Validation and application of a high-performance liquid chromatography-tandem mass spectrometric method for simultaneous quantification of lopinavir and ritonavir in human plasma using semi-automated…
A monolithic-phase based on-line extraction approach for determination of pharmaceutical components in human plasma by HPLC-MS/MS and a comparison with liquid-liquid extraction.