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A novel ether core lipid with H-shaped C80-isoprenoid hydrocarbon chain from the hyperthermophilic methanogen Methanothermus fervidus.
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Characterization and mechanistic study of a radical SAM dehydrogenase in the biosynthesis of butirosin.
TLDR
In vitro assay of the overexpressed enzyme revealed that BtrN catalyzed the oxidation of DOIA under the strictly anaerobic conditions along with consumption of an equimolar amount of SAM to produce 5'-deoxyadenosine, methionine, and 3-amino-2,3-dideoxy-scyllo-inosose (amino -DOI).
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Enzymatic approach to unnatural glycosides with diverse aglycon scaffolds using glycosyltransferase VinC.
Glycosyltransferase VinC was explored for a construction of glycoside libraries using dTDP-vicenisamine and structurally unrelated unnatural aglycons, and new unnatural vicenisaminides were…
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Metabolite profiling of plant carotenoids using the matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
TLDR
It is described how matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI/TOF-MS) can be utilized to acquire mass spectra of carotenoids effectively, which is sensitive, reduces 'spot to spot' variation and provides high mass accuracy, thus aiding identification.
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Butirosin-biosynthetic gene cluster from Bacillus circulans.
TLDR
The gene cluster found so far was determined to be a part of the butirosin biosynthetic gene cluster, which is an interesting 2-deoxystreptamine (DOS)-containing aminoglycoside antibiotic produced by non-actinomycete Bacilli.
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Cloning, sequencing, and functional analysis of the biosynthetic gene cluster of macrolactam antibiotic vicenistatin in Streptomyces halstedii.
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Biosynthesis of archaeal membrane lipids: digeranylgeranylglycerophospholipid reductase of the thermoacidophilic archaeon Thermoplasma acidophilum.
TLDR
The present study clearly demonstrates that 2,3-digeranylgeranylglycerophospholipid reductase is a membrane associated protein and that the hydrogenation of each double bond of 2, 3-di-O-geranylgeranicalglyceryl phosphate is catalyzed by a single enzyme.
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Structure and biosynthesis of FD-594; a new antitumor antibiotic.
The structure of a novel antitumor antibiotics FD-594 (1), produced by Streptomyces sp. TA-0256, was determined to have a glycosylated pyrano[4',3':6,7]naphtho[1,2-b]-xanthene skeleton by means of…
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Mechanistic study on the reaction of a radical SAM dehydrogenase BtrN by electron paramagnetic resonance spectroscopy.
TLDR
Comparison of signals derived from a [4Fe-4S] center of BtrN incubated with various combinations of products (5'-deoxyadenosine, l-methionine, and amino-DOI) and substrates (SAM and DOIA) indicated that the EPR signals observed during the reaction were derived from free BTrN, a Btrn-SAM complex, and a BTRN-SAM-DOIA complex.
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Aglycon switch approach toward unnatural glycosides from natural glycoside with glycosyltransferase VinC
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