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Mitotic chromosome doubling of plant tissues in vitro
In vitro chromosome doubling can be induced by several antimitotic agents. The most commonly used are colchicine, oryzalin and trifluralin. The process of induced chromosome doubling in vitroExpand
In vitro induction of tetraploids in ornamental Ranunculus
This study investigates the capacity of the antimitotic agents colchicine, oryzalin and trifluralin for inducing polyploidisation of Ranunculus asiaticus ‘Alfa’ in vitro shoots. Flow cytometry wasExpand
Occurrence of viable unreduced pollen in a Begonia collection
Polyploidy is widespread in plants and has played a major role in the evolution and diversification of the plant kingdom. Unreduced (2n) gametes are an interesting tool for polyploidisation and theExpand
Progress in plant protoplast research
Abstract In this review we focus on recent progress in protoplast regeneration, symmetric and asymmetric hybridization and novel technology developments. Regeneration of new species and improvedExpand
Application of embryo rescue after interspecific crosses in the genus Rhododendron
Interspecific hybridization between evergreen pot azalea (Tsutsusi) cultivars and genotypes of other Rhododendron subgenera or sections (Rhododendron, Hymenanthes, Pentanthera, Vireya) isExpand
Chemically Induced Polyploidization in Spathiphyllum wallisii Regel through Somatic Embryogenesis
Polyploid Spathiphyllum wallisii Regel 'Speedy' plants were obtained from somatic embryos induced on anther filaments exposed to mitosis inhibitors. Primary embryos yielded less polyploid plantletsExpand
Effect of enzyme concentrations on protoplast isolation and protoplast culture of Spathiphyllum and Anthurium
Vital protoplasts from Spathiphyllum wallisii ‘Alain’ and Anthurium scherzerianum ‘238’ were isolated from both somatic embryos and leaves. The highest yields were obtained when 1.5% cellulase, 0.5%Expand
Meiotic aberrations during 2n pollen formation in Begonia
Unreduced gametes are the driving force for the polyploidization of plants in nature, and are also an important tool for ploidy breeding. The final heterozygosity of a 2n pollen grain depends on theExpand
Induction of homozygous Spathiphyllum wallisii genotypes through gynogenesis
In Spathiphyllum wallisii the production of doubled haploids was attempted. Different combinations of growth regulators were tested, as well as different cultivars. The use of TDZ (0.25–1 μM) inExpand
Plant Tissue Culture Techniques for Breeding
Several tissue culture techniques can be used to generate genetic variation in plants. These in vitro procedures have been applied successfully in many species and have been used to create plantsExpand