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The N-end rule in bacteria.
It is shown that the bacterium Escherichia coli also has the N-end rule pathway, and the adenosine triphosphate-dependent protease Clp (Ti) is required for the degradation of N- end rule substrates in E. coli.
Artificial nucleosome positioning sequences.
The emerging rules for the sequence dependence of DNA bendability are used to design and test a series of DNA molecules that incorporate strongly into nucleosomes and infer that the distortion energy of DNA bending in the nucleosome is only weakly dependent on DNA sequence.
The N-end rule in Escherichia coli: cloning and analysis of the leucyl, phenylalanyl-tRNA-protein transferase gene aat
The cloning and analysis of aat, the Escherichia coli gene that encodes leucyl, phenylalanyl-tRNA-protein transferase (L/F-transferase), a component of the bacterial N-end rule pathway, are reported, suggesting the specificity of L/F -transferase in vivo may be greater than that in vitro.
Effects of DNA sequence and histone-histone interactions on nucleosome placement.
FemABX Family Members Are Novel Nonribosomal Peptidyltransferases and Important Pathogen-specific Drug Targets*
The FemABX family members, a large family of genes previously implicated in interchain peptide synthesis but with unknown specific functions, are ideal candidates for pathogen-specific antibiotic development.
Inactivation of the 20S proteasome in Mycobacterium smegmatis
This work uses a positive/negative selection scheme to inactivate the genes encoding 20S proteasome subunits and demonstrates that, in contrast to eukaryotic cells, M. smegmatis cells lacking intact proteAsome genes are viable and phenotypically indistinguishable from congenic strains containing proteasomes.
Aminoacyl-tRNA Recognition by the Leucyl/Phenylalanyl-tRNA-Protein Transferase*
Deoxyoligonucleotide ·aminoacyl-tRNA hybrids (dO·AA-tRNAs) are employed to characterize the determinants of the Leu-t RNALeu-4 acceptor stem recognized by the L/F-transferase.
Substrate Recognition by the Leucyl/Phenylalanyl-tRNA-protein Transferase
- I. Ichetovkin, G. Abramochkin, T. E. Shrader
- Biology, ChemistryJournal of Biological Chemistry
- 26 December 1997
Data is presented suggesting that the L/F-transferase is not a homolog of the peptidyltransferase enzymes involved in cell wall peptidoglycan biosynthesis in Gram-positive species, such as Staphylococcus aureus.
DNA bending in protein-DNA complexes.
Functional role of the N-terminal region of the Lon protease from Mycobacterium smegmatis.
Lon protease homologues contain a poorly conserved N-terminal region of variable length. To better understand the role of the N-terminal region of Lon in the complicated reaction cycle of…