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Gene cloning, biochemical characterization and physiological role of a thermostable low-specificity L-threonine aldolase from Escherichia coli.
The ltaE gene encoding for a thermostable low-specificity L-threonine aldolase, which catalyzes the cleavage of L-threonine/L-allo-threonine to glycine and acetaldehyde, was cloned from Escherichia…
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Cellulose complementing factor (Ccp) is a new member of the cellulose synthase complex (terminal complex) in Acetobacter xylinum.
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Cloning and nucleotide sequence of the gene responsible for chlorination of tetracycline.
TLDR
A search for sequences homologous to these ORFs found that the former product strongly resembles that of the 6-hydroxylation enzyme for oxytetracycline biosynthesis, and that the latter product has a weak but significant similarity to the hydroxyindole O-methyltransferase of bovine pineal gland.
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The GLY1 gene of Saccharomyces cerevisiae encodes a low-specific L-threonine aldolase that catalyzes cleavage of L-allo-threonine and L-threonine to glycine--expression of the gene in Escherichia…
TLDR
The GLY1 gene was amplified by PCR, with a designed ribosome-binding site, cloned into pUC118, and expressed in Escherichia coli cells, and the enzyme was purified to homogeneity, as judged by polyacrylamide gel electrophoresis.
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Fusicoccins are biosynthesized by an unusual chimera diterpene synthase in fungi
TLDR
It is found that (+)-fusicocca-2,10 (14)-diene, a tricyclic hydrocarbon precursor for fusicoccins, is biosynthesized from the C5 isoprene units by an unusual multifunctional enzyme, P. amygdali fusICoccadiene synthase (PaFS), which shows both prenyltransferase and terpene cyclase activities.
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Reconstitution of biosynthetic machinery for indole-diterpene paxilline in Aspergillus oryzae.
TLDR
The highly intriguing stepwide epoxidation/cyclization mechanism for the construction of core structure has been confirmed and "tandem transformation" to simultaneously introduce two genes using a single vector may provide further option for the reconstitution strategy to synthesize more complex fungal metabolites.
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fldA is an essential gene required in the 2‐C‐methyl‐D‐erythritol 4‐phosphate pathway for isoprenoid biosynthesis
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Gene cloning and overproduction of low-specificity d-threonine aldolase from Alcaligenes xylosoxidans and its application for production of a key intermediate for parkinsonism drug
TLDR
The recombinant low-specificity d-threonine aldolase was shown to be an efficient biocatalyst for resolution of l-β-3,4-methylenedioxyphenylserine, an intermediate for production of a therapeutic drug for Parkinson's disease.
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Gene Cloning, Nucleotide Sequencing, and Purification and Characterization of the Low-Specificityl-Threonine Aldolase from Pseudomonas sp. Strain NCIMB 10558
TLDR
Site-directed mutagenesis experiments showed that substitution of Lys207 with Ala or Arg resulted in a significant loss of enzyme activity, with the corresponding disappearance of the absorption maximum at 420 nm, suggesting that Lys207 of thel-TA probably functions as an essential catalytic residue, forming an internal Schiff base with the pyridoxal 5′-phosphate of the enzyme to catalyze the reversible aldol reaction.
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An Alternative Menaquinone Biosynthetic Pathway Operating in Microorganisms
TLDR
The outline of this alternative pathway in a nonpathogenic strain of Streptomyces is deduced by bioinformatic screening, gene knockouts, shotgun cloning with isolated mutants, and in vitro studies with recombinant enzymes.
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