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Gene cloning, biochemical characterization and physiological role of a thermostable low-specificity L-threonine aldolase from Escherichia coli.
- J. Liu, T. Dairi, N. Itoh, M. Kataoka, S. Shimizu, H. Yamada
- BiologyEuropean journal of biochemistry
- 1 July 1998
The ltaE gene encoding for a thermostable low-specificity L-threonine aldolase, which catalyzes the cleavage of L-threonine/L-allo-threonine to glycine and acetaldehyde, was cloned from Escherichia…
Cellulose complementing factor (Ccp) is a new member of the cellulose synthase complex (terminal complex) in Acetobacter xylinum.
Cloning and nucleotide sequence of the gene responsible for chlorination of tetracycline.
- T. Dairi, T. Nakano, K. Aisaka, R. Katsumata, M. Hasegawa
- Biology, ChemistryBioscience, biotechnology, and biochemistry
- 23 June 1995
A search for sequences homologous to these ORFs found that the former product strongly resembles that of the 6-hydroxylation enzyme for oxytetracycline biosynthesis, and that the latter product has a weak but significant similarity to the hydroxyindole O-methyltransferase of bovine pineal gland.
The GLY1 gene of Saccharomyces cerevisiae encodes a low-specific L-threonine aldolase that catalyzes cleavage of L-allo-threonine and L-threonine to glycine--expression of the gene in Escherichia…
- J. Liu, S. Nagata, T. Dairi, H. Misono, S. Shimizu, H. Yamada
- Biology, ChemistryEuropean journal of biochemistry
- 1 April 1997
The GLY1 gene was amplified by PCR, with a designed ribosome-binding site, cloned into pUC118, and expressed in Escherichia coli cells, and the enzyme was purified to homogeneity, as judged by polyacrylamide gel electrophoresis.
Fusicoccins are biosynthesized by an unusual chimera diterpene synthase in fungi
- T. Toyomasu, Mai Tsukahara, T. Sassa
- BiologyProceedings of the National Academy of Sciences
- 27 February 2007
It is found that (+)-fusicocca-2,10 (14)-diene, a tricyclic hydrocarbon precursor for fusicoccins, is biosynthesized from the C5 isoprene units by an unusual multifunctional enzyme, P. amygdali fusICoccadiene synthase (PaFS), which shows both prenyltransferase and terpene cyclase activities.
Reconstitution of biosynthetic machinery for indole-diterpene paxilline in Aspergillus oryzae.
- Koichi Tagami, Chengwei Liu, H. Oikawa
- Chemistry, BiologyJournal of the American Chemical Society
- 15 January 2013
The highly intriguing stepwide epoxidation/cyclization mechanism for the construction of core structure has been confirmed and "tandem transformation" to simultaneously introduce two genes using a single vector may provide further option for the reconstitution strategy to synthesize more complex fungal metabolites.
fldA is an essential gene required in the 2‐C‐methyl‐D‐erythritol 4‐phosphate pathway for isoprenoid biosynthesis
Gene cloning and overproduction of low-specificity d-threonine aldolase from Alcaligenes xylosoxidans and its application for production of a key intermediate for parkinsonism drug
The recombinant low-specificity d-threonine aldolase was shown to be an efficient biocatalyst for resolution of l-β-3,4-methylenedioxyphenylserine, an intermediate for production of a therapeutic drug for Parkinson's disease.
Gene Cloning, Nucleotide Sequencing, and Purification and Characterization of the Low-Specificityl-Threonine Aldolase from Pseudomonas sp. Strain NCIMB 10558
- Ji-Quan Liu, S. Ito, H. Yamada
- Biology, ChemistryApplied and Environmental Microbiology
- 1 February 1998
Site-directed mutagenesis experiments showed that substitution of Lys207 with Ala or Arg resulted in a significant loss of enzyme activity, with the corresponding disappearance of the absorption maximum at 420 nm, suggesting that Lys207 of thel-TA probably functions as an essential catalytic residue, forming an internal Schiff base with the pyridoxal 5′-phosphate of the enzyme to catalyze the reversible aldol reaction.
An Alternative Menaquinone Biosynthetic Pathway Operating in Microorganisms
The outline of this alternative pathway in a nonpathogenic strain of Streptomyces is deduced by bioinformatic screening, gene knockouts, shotgun cloning with isolated mutants, and in vitro studies with recombinant enzymes.