• Publications
  • Influence
DNA-DNA hybridization values and their relationship to whole-genome sequence similarities.
It is concluded that ANI can accurately replace DDH values for strains for which genome sequences are available and reveal extensive gene diversity within the current concept of "species". Expand
Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates.
In the present study six assays for the quantification of biofilms formed in 96-well microtiter plates were optimised and evaluated: the crystal violet (CV) assay, the Syto9 assay, the fluoresceinExpand
Ralstonia taiwanensis sp. nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient.
A polyphasic taxonomic study was conducted to clarify the relationships of eight isolates from root nodules of Mimosa species and one isolate from sputum of a cystic fibrosis patient, which classified as a novel Ralstonia species, for which the name RAlstonia taiwanensis sp. Expand
Re-evaluating prokaryotic species
The current and future impact of multilocus nucleotide-sequence-based approaches to prokaryotic systematics are discussed and the potential, and difficulties, of assigning species status to biologically or ecologically meaningful sequence clusters are considered. Expand
Diversity and significance of Burkholderia species occupying diverse ecological niches.
An overview of the taxonomic and ecological diversity of the Burkholderia genus with particular emphasis on strains belonging to the Burk holderia cepacia complex is given and the important question whether 'good' and 'bad' strains are actually the same is addressed. Expand
Diagnostically and Experimentally Useful Panel of Strains from the Burkholderia cepacia Complex
A panel of 30 well-characterized strains representative of each genomovar and new species was assembled to assist with identification, epidemiological analysis, and virulence studies on this important group of opportunistic pathogens. Expand
Occurrence of multiple genomovars of Burkholderia cepacia in cystic fibrosis patients and proposal of Burkholderia multivorans sp. nov.
An integrated genotypic and phenotypic analysis of 128 strains of the genera Burkholderia, Ralstonia, and Pseudomonas shows that presumed B. cepacia strains isolated from cystic fibrosis patients belong to at least five distinct genomic species, one of which was identified as Burk holderia vietnamiensis. Expand
Taxonomy of the genus Cupriavidus: a tale of lost and found.
DNA-DNA hybridization experiments and an evaluation of phenotypic characteristics, DNA base ratios and 16S rRNA gene sequences demonstrated that Wautersia eutropha (Davies 1969) Vaneechoutte et al. 2004 is a later synonym of Cupriavidus necator Makkar and Casida 1987, the type species of the genus CupRIavidus. Expand
PCR-Based Assay for Differentiation of Pseudomonas aeruginosa from Other Pseudomonas Species Recovered from Cystic Fibrosis Patients
Two PCR assays are designed: one is specific for the genus Pseudomonas, while the other is Specific for P. aeruginosa, both of which show 100% sensitivity and specificity. Expand
Taxonomy and Identification of the Burkholderia cepacia Complex
At the beginning of this review it is essential to clarify the terminology that will be used to refer to the members of the Burkholderia cepacia complex and their relatives. The name B. cepacia willExpand