• Publications
  • Influence
A helical arch allowing single-stranded DNA to thread through T5 5'-exonuclease
THE 5'-exonucleases are enzymes that are essential for DNA replication and repair1. As well as their exonucleolytic action, removing nucleotides from the 5'-end of nucleic acid molecules such asExpand
  • 177
  • 8
Roles of divalent metal ions in flap endonuclease–substrate interactions
Flap endonucleases (FENs) have essential roles in DNA processing. They catalyze exonucleolytic and structure-specific endonucleolytic DNA cleavage reactions. Divalent metal ions are essentialExpand
  • 52
  • 3
Structure-specific DNA cleavage by 5' nucleases.
Many processes, such as DNA replication, recombination and repair, produce branched DNA structures. These bifurcated molecules are trimmed by a group of homologous 5'-3' exonucleases (also known asExpand
  • 57
  • 2
Mutagenesis of conserved lysine residues in bacteriophage T5 5'-3' exonuclease suggests separate mechanisms of endo-and exonucleolytic cleavage.
Efficient cellular DNA replication requires the activity of a 5'-3' exonuclease. These enzymes are able to hydrolyze DNA.DNA and RNA.DNA substrates exonucleolytically, and they are structure-specificExpand
  • 31
  • 2
  • PDF
The X-ray structure of an atypical homeodomain present in the rat liver transcription factor LFB1/HNF1 and implications for DNA binding.
The transcription factor LFB1/HNF1 from rat liver nuclei is a 628 amino acid protein that functions as a dimer binding to the inverted palindrome GTTAATN-ATTAAC consensus site. We have crystallized aExpand
  • 47
  • 1
Interactions of mutant and wild-type flap endonucleases with oligonucleotide substrates suggest an alternative model of DNA binding
Previous structural studies on native T5 5′ nuclease, a member of the flap endonuclease family of structure-specific nucleases, demonstrated that this enzyme possesses an unusual helical arch mountedExpand
  • 25
  • 1
Prokaryotic 5'-3' exonucleases share a common core structure with gamma-delta resolvase.
The three dimensional crystal structure of T5 5'-3' exonuclease was compared with that of two other members of the 5'-3' exonuclease family: T4 ribonuclease H and the N-terminal domain of ThermusExpand
  • 19
Engineering human MEK-1 for structural studies: A case study of combinatorial domain hunting.
Structural biology studies typically require large quantities of pure, soluble protein. Currently the most widely-used method for obtaining such protein involves the use of bioinformatics andExpand
  • 15
Recollections of the electron crystallographic heavy atom derivative search of purple membrane: the quest for EM structure determination.
  • T. Ceska
  • Medicine, Chemistry
  • Journal of structural biology
  • 1 September 1999
The use of multiple isomorphous replacement in protein electron crystallography for phase determination has been systematically studied only for purple membrane, even though the use of heavy atoms orExpand
  • 2